Population Structure of Candida parapsilosis: No Genetic Difference Between French and Uruguayan Isolates Using Microsatellite Length Polymorphism

Marie Desnos-Ollivier; Victoria Bórmida; Philippe Poirier; Céline Nourrisson; Dinorah Pan; Stéphane Bretagne; Andrès Puime; Françoise Dromer

Population Structure of Candida parapsilosis: No Genetic Difference Between French and Uruguayan Isolates Using Microsatellite Length Polymorphism

Marie Desnos-Ollivier ¹ Victoria Bórmida ^{2, 3} Philippe Poirier ^{4, 5} Céline Nourrisson ^{5, 4} Dinorah Pan ^{2, 3} Stéphane Bretagne ^{1, 6, 7} Andrès Puime ² Françoise Dromer ^{1, *}

* Corresponding author

1 CNRMA - Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire

2 Ministerio de salud publica, Unidad de Parasitologia y Micologia, Montevideo, Uruguay

3 Universidad de Montevideo

4 CHU Clermont-Ferrand

5 LMGE - Laboratoire Microorganismes : Génome et Environnement - Clermont Auvergne

6 UPD7 - Université Paris Diderot - Paris 7

7 Laboratoire de Parasitologie-Mycologie [Paris]

Abstract : Candida parapsilosis is a human commensal yeast, frequently involved in infection worldwide and especially in neonates. It is the second species responsible for bloodstream infections in Uruguay and the third species in France. We were interested in knowing whether the population structure of isolates responsible for candidemia in France and in Uruguay was different. Genotyping methods based on microsatellite length polymorphism (MLP) have been described and are especially used for investigation of local outbreaks. We therefore determined the genotypes of 159 C. parapsilosis isolates recovered from 122 patients (84 French patients from 43 hospitals and 38 Uruguayan patients from 10 hospitals) using three microsatellites markers previously described. Our results confirmed that C. parapsilosis population has a high genetic diversity, clonal inheritance and that majority of patients were infected by a single isolate. But we described recurrent infections due to related or unrelated genotypes resulting from isolates harboring loss or gain of heterozygosity. We also described three cases of coinfections due to unrelated genotypes. We did not uncover geographic specificity but observed two linked genotypes that seem to be associated with voriconazole resistance. Finally, among eight isolates involved in grouped cases, the genotypes were similar in six cases supporting the hypothesis of inter-patient transmission. These results confirmed the usefulness of performing MLP genotyping analysis for grouped cases of C. parapsilosis isolates in order to reinforce preventive hygiene measures.

Keywords : Population structure Microsatellites genotyping Candida parapsilosis Microsatellites genotyping Azoles resistance Candidemia Population structure Azoles resistance Candida parapsilosis Candidemia

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Mycology

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Submitted on : Monday, August 6, 2018 - 2:36:04 PM
Last modification on : Wednesday, July 24, 2019 - 5:18:01 PM

Population Structure of Candida parapsilosis: No Genetic Difference Between French and Uruguayan Isolates Using Microsatellite Length Polymorphism

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Population Structure of Candida parapsilosis: No Genetic Difference Between French and Uruguayan Isolates Using Microsatellite Length Polymorphism

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