One-Step Multiplex PCR Assay for Differentiating Proposed New Species “Clostridium neonatale” from Closely Related Species

Laurent Ferraris (1) , Sophia Schönherr (1) , Philippe Bouvet (2) , Brunhilde Dauphin (3) , Michel Popoff (2) , Marie Jose Butel (1) , Julio Aires (1)

1 EA 4065 - Ecosystème intestinal, probiotiques, antibiotiques
2 Bactéries anaérobies et Toxines
3 Andromas SAS

"Clostridium neonatale" sp. nov., previously involved in an outbreak of neonatal necrotizing enterocolitis, was recently proposed as a new species of the Clostridium genus sensu stricto. We developed a one-step multiplex colony PCR for C. neonatale identification and investigated C. neonatale intestinal colonization frequency in healthy preterm neonates.

Domaines: Sciences du Vivant [q-bio]; Toxicologie

Laurent Ferraris, Sophia Schönherr, Philippe Bouvet, Brunhilde Dauphin, Michel Popoff, et al.. One-Step Multiplex PCR Assay for Differentiating Proposed New Species “Clostridium neonatale” from Closely Related Species. Journal of Clinical Microbiology, 2015, 53 (11), pp.3621 - 3623. ⟨10.1128/JCM.01404-15⟩. ⟨pasteur-01783519⟩