Parallel derivation of isogenic human primed and naive induced pluripotent stem cells - Archive ouverte HAL Access content directly
Journal Articles Nature Communications Year : 2018

Parallel derivation of isogenic human primed and naive induced pluripotent stem cells

(1, 2, 3) , (1, 2, 3, 4) , (1, 2, 3) , (5) , (5) , (1, 2, 3, 6) , (4) , (7) , (7) , (5) , (1, 2, 3) , (1, 2, 3, 6) , (8) , (9) , (1, 2, 3, 6) , (9) , (5) , (10) , (9) , (10, 11) , (12) , (9, 6) , (1, 2, 3, 6) , (4) , (8) , (13) , (13) , (7) , (1, 2, 3, 6) , (1, 2, 3, 5) ,
1
2
3
4
5
6
7
8
9
10
11
12
13
Céline Vallot
Claire Pecqueur
Milieu Intérieur Consortium
  • Function : Author
  • PersonId : 1030290

Abstract

Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of cells of embryonic origin and offer the potential to generate patient-specific pluripotent stem cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, human iPSCs (hiPSCs), restricting their use to post-implantation human development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we develop a method to generate naive hiPSCs directly from somatic cells, using OKMS overexpression and specific culture conditions, further enabling parallel generation of their isogenic primed counterparts. We benchmark naive hiPSCs against human preimplantation epiblast and reveal remarkable concordance in their transcriptome, dependency on mito-chondrial respiration and X-chromosome status. Collectively, our results are essential for the understanding of pluripotency regulation throughout preimplantation development and generate new opportunities for disease modeling and regenerative medicine.
Fichier principal
Vignette du fichier
8_Publication_NatCom-2018-iPSC.pdf (2.79 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-01758726 , version 1 (04-04-2018)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Stéphanie Kilens, Dimitri Meistermann, Diego Moreno, Caroline Chariau, Anne Gaignerie, et al.. Parallel derivation of isogenic human primed and naive induced pluripotent stem cells. Nature Communications, 2018, 9 (1), pp.302 - 314. ⟨10.1038/s41467-017-02107-w⟩. ⟨pasteur-01758726⟩
843 View
530 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More