Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Parallel derivation of isogenic human primed and naive induced pluripotent stem cells

Stéphanie Kilens 1, 2, 3 Dimitri Meistermann 1, 2, 3, 4 Diego Moreno 1, 2, 3 Caroline Chariau 5 Anne Gaignerie 5 Arnaud Reignier 1, 2, 3, 6 yohann Lelièvre 4 Miguel Casanova 7 Céline Vallot 7 Steven Nedellec 5 Léa Flippe 1, 2, 3 Julie Firmin 1, 2, 3, 6 Juan Song 8 Eric Charpentier 9 Jenna Lammers 1, 2, 3, 6 Audrey Donnart 9 Nadège Marec 5 Wallid Deb 10 Audrey Bihouée 9 Cédric Le Caignec 10, 11 Claire Pecqueur 12 Richard Redon 9, 6 Paul Barriere 1, 2, 3, 6 Jérémie Bourdon 4 Vincent Pasque 8 Magali Soumillon 13 Tarjei Mikkelsen 13 Claire Rougeulle 7 Thomas Freour 1, 2, 3, 6 Laurent David 1, 2, 3, 5 Milieu Intérieur Consortium 
Abstract : Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of cells of embryonic origin and offer the potential to generate patient-specific pluripotent stem cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, human iPSCs (hiPSCs), restricting their use to post-implantation human development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we develop a method to generate naive hiPSCs directly from somatic cells, using OKMS overexpression and specific culture conditions, further enabling parallel generation of their isogenic primed counterparts. We benchmark naive hiPSCs against human preimplantation epiblast and reveal remarkable concordance in their transcriptome, dependency on mito-chondrial respiration and X-chromosome status. Collectively, our results are essential for the understanding of pluripotency regulation throughout preimplantation development and generate new opportunities for disease modeling and regenerative medicine.
Complete list of metadata

Cited literature [63 references]  Display  Hide  Download
Contributor : Milieu Intérieur Consortium Connect in order to contact the contributor
Submitted on : Wednesday, April 4, 2018 - 5:08:59 PM
Last modification on : Wednesday, April 27, 2022 - 3:51:28 AM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License



Stéphanie Kilens, Dimitri Meistermann, Diego Moreno, Caroline Chariau, Anne Gaignerie, et al.. Parallel derivation of isogenic human primed and naive induced pluripotent stem cells. Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.302 - 314. ⟨10.1038/s41467-017-02107-w⟩. ⟨pasteur-01758726⟩



Record views


Files downloads