Serotype Distribution of Clinical Streptococcus pneumoniae Isolates before the Introduction of the 13-Valent Pneumococcal Conjugate Vaccine in Cambodia

Malin Inghammar (1, 2) , Youlet By (3, 4) , Christina Farris (5) , Thong Phe (6) , Laurence Borand (1) , Alexandra Kerleguer (1) , Sophie Goyet (1) , Vonthanak Saphonn (4) , Chanleakhena Phoeung (4) , Sirenda Vong (1) , Blandine Rammaert (7, 8) , Charles Mayaud (1) , Bertrand Guillard (1) , Chadwick Yasuda (5) , Matthew Kasper (5) , Gavin Ford (5) , Steven Newell (5) , Ung Sam An (9) , Buth Sokhal (9) , Sok Touch (10) , Paul Turner (11, 12) , Jan Jacobs (6, 13, 14) , Mélina Messaoudi (15) , Florence Komurian-Pradel (15) , Arnaud Tarantola (1)

1 Institut Pasteur du Cambodge
2 Skane University Hospital [Lund]
3 Fondation Mérieux
4 University of Health Science
5 US Naval Medical Research Unit n°2
6 Shihanouk Hospital Centre of HOPE
7 Médecine Interne et Maladies Infectieuses, CHU de Poitiers, Poitiers, France
8 PHAR - Pharmacologie des anti-infectieux
9 Cambodian National Laboratory of Public Health
10 CDC MOH - Communicable Disease Department [Phnom Penh]
11 Cambodia Oxford Medical Research
12 Nuffield Department of Medicine
13 ITM - Institute of Tropical Medicine [Antwerp]
14 KU Leuven - Catholic University of Leuven - Katholieke Universiteit Leuven
15 CIRI - Centre International de Recherche en Infectiologie

Childhood vaccination with the 13-valent pneumococcal conjugate vaccine (PCV13) was introduced in Cambodia in January 2015. Baseline data regarding circulating serotypes are scarce. All microbiology laboratories in Cambodia were contacted for identification of stored isolates ofStreptococcus pneumoniaefrom clinical specimens taken before the introduction of PCV13. Available isolates were serotyped using a multiplex polymerase chain reaction method. Among 166 identified isolates available for serotyping from patients with pneumococcal disease, 4% were isolated from upper respiratory samples and 80% were from lower respiratory samples, and 16% were invasive isolates. PCV13 serotypes accounted for 60% (95% confidence interval [CI] 52-67) of all isolates; 56% (95% CI 48-64) of noninvasive and 77% (95% CI 57-89) of invasive isolates. Antibiotic resistance was more common among PCV13 serotypes. This study of clinicalS. pneumoniaeisolates supports the potential for high reduction in pneumococcal disease burden and may serve as baseline data for future monitoring ofS. pneumoniaeserotypes circulation after implementation of PCV13 childhood vaccination in Cambodia.

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Human health and pathology; Infectious diseases; Life Sciences [q-bio]; Immunology; Vaccinology

Malin Inghammar, Youlet By, Christina Farris, Thong Phe, Laurence Borand, et al.. Serotype Distribution of Clinical Streptococcus pneumoniae Isolates before the Introduction of the 13-Valent Pneumococcal Conjugate Vaccine in Cambodia. American Journal of Tropical Medicine and Hygiene, 2018, 98 (3), pp.791 - 796. ⟨10.4269/ajtmh.17-0692⟩. ⟨pasteur-01739337⟩