Shuffling of DNA cassettes in a synthetic integron

David Bikard (1) , Didier Mazel (1)

1 PGB - Plasticité du Génome Bactérien - Bacterial Genome Plasticity

The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle genetic elements at a chromosomal locus in E. coli.

Keywords: Synthetic biology; Directed evolution; Recombination; DNA shuffling; Integron; Metabolic engineering

Domains: Life Sciences [q-bio]

David Bikard, Didier Mazel. Shuffling of DNA cassettes in a synthetic integron. Karen M. Polizzi; Cleo Kontoravdi. Synthetic Biology, 1073, Humana Press, pp.169-174, 2013, Methods in Molecular Biology (MIMB), 978-1-62703-624-5 (Hardcover), 978-1-4939-6286-0 (Softcover), 978-1-62703-625-2 (eBook). ⟨10.1007/978-1-62703-625-2_14⟩. ⟨pasteur-01698800⟩