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Shuffling of DNA cassettes in a synthetic integron
Abstract : The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle genetic elements at a chromosomal locus in E. coli.
Cited literature [12 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01698800
Contributor : Isabelle Hoffmann Connect in order to contact the contributor
Submitted on : Thursday, February 1, 2018 - 4:09:38 PM
Last modification on : Monday, November 22, 2021 - 4:14:05 PM
Long-term archiving on: : Wednesday, May 2, 2018 - 6:30:07 PM
Contributor : Isabelle Hoffmann Connect in order to contact the contributor
Submitted on : Thursday, February 1, 2018 - 4:09:38 PM
Last modification on : Monday, November 22, 2021 - 4:14:05 PM
Long-term archiving on: : Wednesday, May 2, 2018 - 6:30:07 PM
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Methods Mol Bio_Aug 2013.pdf
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