Skip to Main content Skip to Navigation
Book sections

Shuffling of DNA cassettes in a synthetic integron

Abstract : The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle genetic elements at a chromosomal locus in E. coli.
Document type :
Book sections
Complete list of metadatas

Cited literature [12 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01698800
Contributor : Isabelle Hoffmann <>
Submitted on : Thursday, February 1, 2018 - 4:09:38 PM
Last modification on : Monday, January 13, 2020 - 5:08:17 PM
Long-term archiving on: : Wednesday, May 2, 2018 - 6:30:07 PM

File

Methods Mol Bio_Aug 2013.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

David Bikard, Didier Mazel. Shuffling of DNA cassettes in a synthetic integron. Karen M. Polizzi; Cleo Kontoravdi. Synthetic Biology, 1073, Humana Press, pp.169-174, 2013, Methods in Molecular Biology (MIMB), 978-1-62703-624-5 (Hardcover), 978-1-4939-6286-0 (Softcover), 978-1-62703-625-2 (eBook). ⟨10.1007/978-1-62703-625-2_14⟩. ⟨pasteur-01698800⟩

Share

Metrics

Record views

220

Files downloads

273