Tectonic conformational changes of a coronavirus spike glycoprotein promote membrane fusion

Alexandra Walls 1 M. Alejandra Tortorici 2 Joost Snijder 1 Xiaoli Xiong 1 Berend-Jan Bosch 3 Félix A. Rey 2 David Veesler 1, *
* Auteur correspondant
1 Department of Biochemistry [Washington ]
2 Virologie Structurale
3 Utrecht University [Utrecht]
Abstract : The tremendous pandemic potential of coronaviruses was demonstrated twice in the past few decades by two global outbreaks of deadly pneumonia. The coronavirus spike (S) glycoprotein initiates infection by promoting fusion of the viral and cellular membranes through conformational changes that remain largely uncharacterized. Here we report the cryoEM structure of a coronavirus S glycoprotein in the postfusion state, showing large-scale secondary, tertiary, and quaternary rearrangements compared with the prefusion trimer and rationalizing the free-energy landscape of this conformational machine. We also biochemically characterized the molecular events associated with refolding of the metastable prefusion S glycoprotein to the postfusion conformation using limited proteolysis, mass spectrometry, and single-particle EM. The observed similarity between postfusion coronavirus S and paramyxovirus F structures demonstrates that a conserved refolding trajectory mediates entry of these viruses and supports the evolutionary relatedness of their fusion subunits. Finally, our data provide a structural framework for understanding the mode of neutralization of antibodies targeting the fusion machinery and for engineering next-generation subunit vaccines or inhibitors against this medically important virus family.
Keywords : coronavirus cryoEM fusion proteins membrane fusion proteolytic activation
Type de document :
Article dans une revue
Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2017, 114 (42), pp.11157 - 11162. 〈10.1073/pnas.1708727114〉
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Contributeur : M. Alejandra Tortorici <>
Soumis le : jeudi 14 décembre 2017 - 17:29:52
Dernière modification le : mercredi 21 novembre 2018 - 17:36:01

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Alexandra Walls, M. Alejandra Tortorici, Joost Snijder, Xiaoli Xiong, Berend-Jan Bosch, et al.. Tectonic conformational changes of a coronavirus spike glycoprotein promote membrane fusion. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2017, 114 (42), pp.11157 - 11162. 〈10.1073/pnas.1708727114〉. 〈pasteur-01664354〉

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