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Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes.

Abstract : Numerous studies have focused on characterizing and monitoring antigen-specific T cells during the course of an immune response. Mostly indirect methods were used to circumvent the low frequency of T cell precursors and the inherent complexity of T cell receptor (TcR)-MHC-peptide interactions. Here, we took advantage of peptide-specific adhesion induced by immobilized MHC-peptide complexes. We describe a simple technique which allows enrichment in antigen-specific T lymphocytes among a heterogeneous CD8+ T cell population. Enrichment of T cells according to their specificity should facilitate their characterization and provide an attractive tool for immunotherapy.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01659378
Contributor : Nathalie Pardigon <>
Submitted on : Friday, December 8, 2017 - 12:49:48 PM
Last modification on : Thursday, May 28, 2020 - 11:16:03 AM

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Philippe Bousso, Frédérique Michel, Nathalie Pardigon, Nadege Bercovici, Roland Liblau, et al.. Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes.. Immunology Letters, Elsevier, 1997, 59 (2), pp.85-91. ⟨10.1016/S0165-2478(97)00105-3⟩. ⟨pasteur-01659378⟩

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