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Journal articles
Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes.
Abstract : Numerous studies have focused on characterizing and monitoring antigen-specific T cells during the course of an immune response. Mostly indirect methods were used to circumvent the low frequency of T cell precursors and the inherent complexity of T cell receptor (TcR)-MHC-peptide interactions. Here, we took advantage of peptide-specific adhesion induced by immobilized MHC-peptide complexes. We describe a simple technique which allows enrichment in antigen-specific T lymphocytes among a heterogeneous CD8+ T cell population. Enrichment of T cells according to their specificity should facilitate their characterization and provide an attractive tool for immunotherapy.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01659378
Contributor : Nathalie Pardigon Connect in order to contact the contributor
Submitted on : Friday, December 8, 2017 - 12:49:48 PM
Last modification on : Monday, November 22, 2021 - 1:12:04 PM
Contributor : Nathalie Pardigon Connect in order to contact the contributor
Submitted on : Friday, December 8, 2017 - 12:49:48 PM
Last modification on : Monday, November 22, 2021 - 1:12:04 PM
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