N-terminomics identifies Prli42 as a membrane miniprotein conserved in Firmicutes and critical for stressosome activation in Listeria monocytogenes.

Abstract : To adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. In Gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma B regulon. The stressosome is structurally well-characterized in Bacillus, but how it senses stress remains elusive. Here, we report a genome-wide N-terminomic approach in Listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniproteins, among which one, Prli42, is conserved in Firmicutes. Prli42 is membrane-anchored and interacts with orthologues of Bacillus stressosome components. We reconstituted the Listeria stressosome in vitro and visualized its supramolecular structure by electron microscopy. Analysis of a series of Prli42 mutants demonstrated that Prli42 is important for sigma B activation, bacterial growth following oxidative stress and for survival in macrophages. Taken together, our N-terminonic approach unveiled Prli42 as a long-sought link between stress and the stressosome.
Keywords : Pathogens Proteomics
Type de document :
Article dans une revue
Nature Microbiology, Nature Publishing Group, 2017, 2, pp.17005. 〈10.1038/nmicrobiol.2017.5〉
Liste complète des métadonnées

https://hal-pasteur.archives-ouvertes.fr/pasteur-01574963
Contributeur : Marie-Therese Vicente <>
Soumis le : jeudi 17 août 2017 - 09:40:28
Dernière modification le : mardi 13 février 2018 - 01:00:44

Lien texte intégral

Identifiants

Collections

Citation

Francis Impens, Nathalie Rolhion, Lilliana Radoshevich, Christophe Bécavin, Mélodie Duval, et al.. N-terminomics identifies Prli42 as a membrane miniprotein conserved in Firmicutes and critical for stressosome activation in Listeria monocytogenes.. Nature Microbiology, Nature Publishing Group, 2017, 2, pp.17005. 〈10.1038/nmicrobiol.2017.5〉. 〈pasteur-01574963〉

Partager

Métriques

Consultations de la notice

191