Screening test for neutralizing antibodies against yellow fever virus, based on a flavivirus pseudotype

Abstract : Given the possibility of yellow fever virus reintroduction in epidemiologically receptive geographic areas, the risk of vaccine supply disruption is a serious issue. New strategies to reduce the doses of injected vaccines should be evaluated very carefully in terms of immunogenicity. The plaque reduction test for the determination of neutralizing antibodies (PRNT) is particularly time-consuming and requires the use of a confinement laboratory. We have developed a new test based on the use of a non-infectious pseudovirus (WN/YF17D). The presence of a reporter gene allows sensitive determination of neutralizing antibodies by flow cytometry. This WN/YF17D test was as sensitive as PRNT for the follow-up of yellow fever vaccinees. Both tests lacked specificity with sera from patients hospitalized for acute Dengue virus infection. Conversely, both assays were strictly negative in adults never exposed to flavivirus infection or vaccination, and in patients sampled some time after acute Dengue infection. This WN/YF17D test will be particularly useful for large epidemiological studies and for screening for neutralizing antibodies against yellow fever virus.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2017, 12 (5), pp.e0177882. 〈10.1371/journal.pone.0177882〉
Liste complète des métadonnées

Littérature citée [22 références]  Voir  Masquer  Télécharger

https://hal-pasteur.archives-ouvertes.fr/pasteur-01570232
Contributeur : Floran Bidault <>
Soumis le : vendredi 28 juillet 2017 - 16:21:13
Dernière modification le : jeudi 15 mars 2018 - 10:12:30

Fichier

journal.pone.0177882.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Séverine Mercier-Delarue, Christine Durier, N.C. De Verdière, Jean-Dominique Poveda, Vincent Meiffrédy, et al.. Screening test for neutralizing antibodies against yellow fever virus, based on a flavivirus pseudotype. PLoS ONE, Public Library of Science, 2017, 12 (5), pp.e0177882. 〈10.1371/journal.pone.0177882〉. 〈pasteur-01570232〉

Partager

Métriques

Consultations de la notice

37

Téléchargements de fichiers

15