Imaging and analyzing primary cilia in cardiac cells.

Nicolas Diguet; Jean-François Le Garrec; Tommaso Lucchesi; Sigolène M Meilhac

doi:10.1016/bs.mcb.2015.01.008

Journal articles

Imaging and analyzing primary cilia in cardiac cells.

Nicolas Diguet ¹ Jean-François Le Garrec ¹ Tommaso Lucchesi ^{2, 1} Sigolène M Meilhac ^{1, *}

* Corresponding author

1 Cellules Souches et Développement

2 UPMC - Université Pierre et Marie Curie - Paris 6

Abstract : The primary cilium is a small sensory organelle that is required for different aspects of embryonic development, including the formation of the heart. The structure and composition of cilia have been extensively studied, so that several markers of primary cilia have now been identified. However, the role of cilia in specific cell types remains poorly understood. We describe here a series of approaches to image primary cilia in the rodent heart or in primary cultures of cells dissociated from the heart. As the cilium is a marker of cell polarity, we also provide, for quantitative image analysis of cilium orientation, tools which are generally applicable to other types of tissues.

Keywords : Cardiac cell dissociation Cardiac primary cilia Cardiomyocytes Cilium orientation Heart development Histology of the heart Image analysis Primary culture of cardiac cells

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Cellular Biology / Subcellular Processes [q-bio.SC]

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Imaging and analyzing primary cilia in cardiac cells.

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Imaging and analyzing primary cilia in cardiac cells.

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