Imaging and analyzing primary cilia in cardiac cells.

Nicolas Diguet 1 Jean-François Le Garrec 1 Tommaso Lucchesi 2, 1 Sigolène M Meilhac 1, *
* Auteur correspondant
1 Cellules Souches et Développement
2 UPMC - Université Pierre et Marie Curie - Paris 6
Abstract : The primary cilium is a small sensory organelle that is required for different aspects of embryonic development, including the formation of the heart. The structure and composition of cilia have been extensively studied, so that several markers of primary cilia have now been identified. However, the role of cilia in specific cell types remains poorly understood. We describe here a series of approaches to image primary cilia in the rodent heart or in primary cultures of cells dissociated from the heart. As the cilium is a marker of cell polarity, we also provide, for quantitative image analysis of cilium orientation, tools which are generally applicable to other types of tissues.
Keywords : Cardiac cell dissociation Cardiac primary cilia Cardiomyocytes Cilium orientation Heart development Histology of the heart Image analysis Primary culture of cardiac cells
Type de document :
Article dans une revue
Methods in Cell Biology, Elsevier, 2015, Methods in Cilia & Flagella, 127, Chapter 4, p55-73. 〈10.1016/bs.mcb.2015.01.008〉
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01570157
Contributeur : Jeanne Tarabella <>
Soumis le : vendredi 28 juillet 2017 - 15:13:37
Dernière modification le : lundi 17 décembre 2018 - 01:24:38

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PASTEUR | UPMC | SORBONNE-UNIVERSITE

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Nicolas Diguet, Jean-François Le Garrec, Tommaso Lucchesi, Sigolène M Meilhac. Imaging and analyzing primary cilia in cardiac cells.. Methods in Cell Biology, Elsevier, 2015, Methods in Cilia & Flagella, 127, Chapter 4, p55-73. 〈10.1016/bs.mcb.2015.01.008〉. 〈pasteur-01570157〉

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