Identification of Lipoproteins Using Globomycin and Radioactive Palmitate.

Nienke Buddelmeijer (1)

1 BGPB - Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall

Bacterial lipoproteins are characterized by fatty acids that are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Three enzymatic steps are involved in the synthesis of mature triacylated lipoprotein: prolipoprotein converts into diacylglyceryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the detection of one of these intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using (3)H-palmitate labeling and inhibition by globomycin and detection by fluorography.

Keywords: 3H-palmitate labeling; Fluorography; Globomycin; Tris-tricine gel electrophoresis

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Nienke Buddelmeijer. Identification of Lipoproteins Using Globomycin and Radioactive Palmitate.. Laure Journet et Eric Cascales Bacterial Protein Secretion Systems, 1615, Humana Press, pp.75-80, 2017, Methods in Molecular Biology, 978-1-4939-7033-9. ⟨10.1007/978-1-4939-7033-9_5⟩. ⟨pasteur-01561093⟩