Studying the Immune Synapse in HIV-1 Infection.

Iratxe del Río-Iñiguez (1) , Jérôme Bouchet (1) , Andrés Alcover (1)

1 Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology

T cells are the main cellular targets of the human immunodeficiency virus 1 (HIV-1). HIV-1 infection induces pleiotropic effects on the infected T cell that modify the T cell capacity to respond to antigen and facilitates virus replication. HIV-1 infection subverts the formation and function of the immunological synapse altering both actin cytoskeleton remodeling and intracellular vesicle traffic. We describe here our methods to unveil how HIV-1 and in particular its protein Nef modify vesicle traffic to the immunological synapse, perturbing the synapse activation capacity.

Keywords: Endosomes; HIV-1; Immunological synapse; Nef; T cell activation; TCR signaling; Vesicle traffic; Cytoskeleton

Domains: Life Sciences [q-bio]; Cellular Biology; Cell Behavior [q-bio.CB]

Iratxe del Río-Iñiguez, Jérôme Bouchet, Andrés Alcover. Studying the Immune Synapse in HIV-1 Infection.. Methods in Molecular Biology, Volume 1584, pp.545-557, 2017, springer protocols - The Immune Synapse : methods and protocols, ⟨10.1007/978-1-4939-6881-7_34⟩. ⟨pasteur-01519883⟩