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Journal articles
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
Ai Ing Li
1, 2
Yan Li
2
Silvia Lopez-Lastra
3, 2
Ralph Stadhouders
4
Franziska Paul
5
Armanda Casrouge
2
Nicolas Serafini
2
Anne Puel
6
Jacinta Bustamante
6
Laura Surace
2
Guillemette Masse-Ranson
2
Eyal David
5
Helene Strick-Marchand
2
Lionel Le Bourhis
7
Roberto Cocchi
8
Davide Topazio
8
Paolo Graziano
8
Lucia Anna Muscarella
8
Lars Rogge
9
Xavier Norel
10
Jean-Michel Sallenave
1
Matthieu Allez
11, 7
Thomas Graf
4
Rudi W Hendriks
12
Jean-Laurent Casanova
13, 6, 14, 15
Ido Amit
5
Hans Yssel
16
James P Di Santo
17, *
*
Corresponding author
Jacinta Bustamante 6
Author
Laura Surace 2
Author
Guillemette Masse-Ranson 2
Author
Eyal David 5
Author
Helene Strick-Marchand 2
Author
Lionel Le Bourhis 7
Author
Roberto Cocchi 8
Author
Davide Topazio 8
Author
Jean-Michel Sallenave 1
Author
Matthieu Allez 11, 7
Author
Thomas Graf 4
Author
Rudi W Hendriks 12
Author
Jean-Laurent Casanova 13, 6, 14, 15
Author
Ido Amit 5
Author
Hans Yssel 16
Author
James P Di Santo 17
Correspondent author
1
UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
2
Immunité Innée (Département d'Immunologie, 25-28 rue du Docteur Roux, 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1223 (101, rue de Tolbiac, 75013 Paris - France)
3
UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
4
BIST - Barcelona Institute of Science and Technology (Carrer Comte d'Urgell, 187, 08036 Barcelona, Espagne - Spain)
5
Weizmann Institute of Science [Rehovot, Israël] (234 Herzl Street, Rehovot 7610001 - Israel)
6
IMAGINE - U1163 - Imagine - Institut des maladies génétiques (IHU Imagine,
156 rue de Vaugirard, 75015 PARIS
et
24 Boulevard du Montparnasse, 75015 Paris - France)
- CNRS - Centre National de la Recherche Scientifique (France)
- UPD5 - Université Paris Descartes - Paris 5 : UMR_S 1163 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1163 (101, rue de Tolbiac, 75013 Paris - France)
7
IUH - Institut Universitaire d'Hématologie (Université Paris Diderot, 1 Av. Claude Vellefaux 75010 Paris - France)
- UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
8
IRCCS "Casa Sollievo della Soffernza" (France)
9
Immunorégulation (Département d'Immunologie, 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA1961 (France)
10
LVTS (UMR_S_1148 / U1148) - Laboratoire de Recherche Vasculaire Translationnelle (Bâtiment INSERM - 46 rue Henri Huchard 75018 Paris - France)
- UP13 - Université Paris 13 (France)
- UPD7 - Université Paris Diderot - Paris 7 : UMR_S_1148 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1148 (101, rue de Tolbiac, 75013 Paris - France)
11
Hôpital Saint-Louis (1, avenue Claude-Vellefaux 75475 PARIS Cedex 10 - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
12
Erasmus MC - Erasmus University Medical Center [Rotterdam] (Rotterdam - Netherlands)
13
CHU Necker - Enfants Malades [AP-HP] (149 Rue de Sèvres 75015 Paris - France)
14
St. Giles Laboratory of Human Genetics of Infectious Diseases (1230 York Ave, New York, NY 10065 - United States)
- Rockefeller University [New York] (1230 York Ave, New York, NY 10065, États-Unis - United States)
15
Howard Hughes Medical Institute (Bronx, New York 10461 - United States)
- Albert Einstein College of Medicine [New York] (300 Morris Park Avenue Bronx, NY 10461 - United States)
16
CIMI - Centre d'Immunologie et de Maladies Infectieuses (91 Boulevard de l'hôpital 75013 Paris - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1135 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : ERL8255 (France)
17
Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
Abstract : Innate lymphoid cells (ILCs) represent innate versions of T helper and cytotoxic T cells that differentiate from committed ILC precursors (ILCPs). How ILCPs give rise to mature tissue-resident ILCs remains unclear. Here, we identify circulating and tissue ILCPs in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration. Human ILCPs robustly generate all ILC subsets in vitro and in vivo. While human ILCPs express low levels of retinoic acid receptor (RAR)-related orphan receptor C (RORC) transcripts, these cells are found in RORC-deficient patients and retain potential for EOMES(+) natural killer (NK) cells, interferon gamma-positive (IFN-γ(+)) ILC1s, interleukin (IL)-13(+) ILC2s, and for IL-22(+), but not for IL-17A(+) ILC3s. Our results support a model of tissue ILC differentiation ("ILC-poiesis"), whereby diverse ILC subsets are generated in situ from systemically distributed ILCPs in response to local environmental signals.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01501939
Contributor : James Di Santo <>
Submitted on : Thursday, May 18, 2017 - 2:39:20 PM
Last modification on : Monday, December 14, 2020 - 3:41:00 PM
Contributor : James Di Santo <>
Submitted on : Thursday, May 18, 2017 - 2:39:20 PM
Last modification on : Monday, December 14, 2020 - 3:41:00 PM
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Identifiers
- HAL Id : pasteur-01501939, version 1
- DOI : 10.1016/j.cell.2017.02.021
- PUBMED : 28283063
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PASTEUR | CNRS | UNIV-PARIS5 | UNIV-PARIS7 | UPMC | CIMI | UNIV-PARIS13 | USPC | ATP-IMMUN | LVTS | SORBONNE-UNIVERSITE | SU-MEDECINE | UNIV-PARIS | IRISA_UBS | UBS | UP-SANTE | SU-TI
Citation
Ai Ing Li, Yan Li, Silvia Lopez-Lastra, Ralph Stadhouders, Franziska Paul, et al.. Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.. Cell, Elsevier, 2017, 168 (6), pp.1086-1100.e10. ⟨10.1016/j.cell.2017.02.021⟩. ⟨pasteur-01501939⟩
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