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Journal articles
3'-5' phosphoadenosine phosphate is an inhibitor of PARP-1 and a potential mediator of the lithium-dependent inhibition of PARP-1 in vivo.
Abstract : pAp (3'-5' phosphoadenosine phosphate) is a by-product of sulfur and lipid metabolism and has been shown to have strong inhibitory properties on RNA catabolism. In the present paper we report a new target of pAp, PARP-1 [poly(ADP-ribose) polymerase 1], a key enzyme in the detection of DNA single-strand breaks. We show that pAp can interact with PARP-1 and inhibit its poly(ADP-ribosyl)ation activity. In vitro, inhibition of PARP-1 was detectable at micromolar concentrations of pAp and altered both PARP-1 automodification and heteromodification of histones. Analysis of the kinetic parameters revealed that pAp acted as a mixed inhibitor that modulated both the Km and the Vmax of PARP-1. In addition, we showed that upon treatment with lithium, a very potent inhibitor of the enzyme responsible for pAp recycling, HeLa cells exhibited a reduced level of poly(ADP-ribosyl)ation in response to oxidative stress. From these results, we propose that pAp might be a physiological regulator of PARP-1 activity.
Cited literature [40 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01427528
Contributor : Undine Mechold Connect in order to contact the contributor
Submitted on : Thursday, January 5, 2017 - 5:40:41 PM
Last modification on : Thursday, November 25, 2021 - 4:00:02 PM
Long-term archiving on: : Thursday, April 6, 2017 - 2:46:39 PM
Contributor : Undine Mechold Connect in order to contact the contributor
Submitted on : Thursday, January 5, 2017 - 5:40:41 PM
Last modification on : Thursday, November 25, 2021 - 4:00:02 PM
Long-term archiving on: : Thursday, April 6, 2017 - 2:46:39 PM
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PARPElie2012.pdf
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