A new sensitive indicator cell line reveals cross-transactivation of the viral LTR by gorilla and chimpanzee simian foamy viruses

Abstract : The majority of currently identified simian foamy virus (SFV)-infected Cameroonian and Gabonese individuals harbor SFV from the gorilla lineage. We constructed an indicator cell line for the quantification of gorilla SFVs, in which the U3 sequence of a gorilla SFV directs the expression of the β-galactosidase protein. The gorilla foamy virus activated β-galactosidase (GFAB) cells efficiently quantified two zoonotic primary gorilla isolates and SFVs from three chimpanzee subspecies. Primary gorilla SFVs replicated more slowly and at lower levels than primary chimpanzee SFVs. Analysis of previously described motifs of Tas proteins and U3 LTRs involved in viral gene synthesis revealed conservation of such motifs in Tas proteins from gorilla and chimpanzee SFVs, but little sequence homology in the LTR regions previously shown to interact with viral and cellular factors.
Liste complète des métadonnées

Littérature citée [55 références]  Voir  Masquer  Télécharger

https://hal-pasteur.archives-ouvertes.fr/pasteur-01414639
Contributeur : Florence Buseyne <>
Soumis le : vendredi 16 décembre 2016 - 18:27:23
Dernière modification le : mercredi 21 mars 2018 - 18:57:49
Document(s) archivé(s) le : mardi 21 mars 2017 - 04:15:41

Fichier

VIRO-16-284R1.pdf
Fichiers produits par l'(les) auteur(s)

Licence


Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales 4.0 International License

Identifiants

Collections

Citation

Caroline Lambert, Réjane Rua, Antoine Gessain, Florence Buseyne. A new sensitive indicator cell line reveals cross-transactivation of the viral LTR by gorilla and chimpanzee simian foamy viruses. Virology, Elsevier, 2016, 496, pp.219 - 226. 〈10.1016/j.virol.2016.06.010〉. 〈pasteur-01414639〉

Partager

Métriques

Consultations de la notice

176

Téléchargements de fichiers

99