Skip to Main content Skip to Navigation
Journal articles

Performance evaluation of multiplex PCR including Aspergillus-not so simple!

Abstract : Multiplex PCRs have been designed for including species other than Aspergillus fumigatus for the diagnosis of invasive aspergillosis, such as microarrays, liquid-phase array, and electrospray-ionization mass spectrometry (PCR/ESI MS). These methods are based on the selection of multiple primers to amplify different species with the specificity checked by hybridization to a probe or by base composition of the amplicon for the PCR/ESI MS. When testing complex samples such as respiratory specimens, some clinically relevant species can be missed. Indeed, it is impossible to design primers able to amplify all the known fungal species with the same efficiency. Therefore, the best amplified species may not be the most clinically relevant. Multiplex assays have also been proposed to detect A. fumigatus DNA and azole resistance. Since the gene responsible for azole resistance is single copy and the gene used for detection is multicopy, only the high fungal loads can be evaluated. Thus, although interesting for investigating mycobiome, the multiplex assays should be used with cautious for the diagnosis of IA or the detection of resistance. For the diagnosis of invasive aspergillosis, validated quantitative PCRs specifically targeting A. fumigatus or a limited set of species to increase sensitivity is a safer option.
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01405214
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 4:14:10 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:12 AM

Links full text

Identifiers

Collections

Citation

Alexandre Alanio, Stéphane Bretagne. Performance evaluation of multiplex PCR including Aspergillus-not so simple!. Medical Mycology, 2016, ⟨10.1093/mmy/myw080⟩. ⟨pasteur-01405214⟩

Share

Metrics

Record views

253