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Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

Abstract : Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01405204
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 4:13:47 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:12 AM

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Alexandre Alanio, Aude Sturny-Leclère, Marion Benabou, Nicolas Guigue, Stéphane Bretagne. Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR. Journal of Microbiological Methods, Elsevier, 2016, 127, pp.160--163. ⟨10.1016/j.mimet.2016.06.015⟩. ⟨pasteur-01405204⟩

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