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Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli.

Darragh Duffy 1 Vincent Rouilly 2, 3 Valentina Libri 3 Milena Hasan Benoit Beitz 3 Mikael David 4 Alejandra Urrutia 5 Aurélie Bisiaux 6 Samuel T Labrie 7 Annick Dubois 8 Ivo G Boneca 9 Cécile Delval 10 Stéphanie Thomas 11 Lars Rogge 3, 12 Manfred Schmolz 7 Lluis Quintana-Murci 13 Matthew L Albert 14 Laurent Abel 15, 16, 17 Andres Alcover 18 Philippe Bousso 19 Ana Cumano 20, 21 Marc Daëron 22 Caroline Demangel 23 Ludovic Deriano 24, 11 James Di santo Françoise Dromer 25, 26 Gérard Eberl 27 Jost Enninga 28 Antonio Freitas Ivo Gomperts-Boneca Serge Hercberg 29 Olivier Lantz Claude Leclerc 30 Hugo Mouquet 31 Sandra Pellegrini 32 Stanislas Pol 33, 1 Anavaj Sakuntabhai 34 Olivier Schwartz 35 Benno Schwikowski 36 Spencer Shorte 37 Vassili Soumelis 38 Frédéric Tangy 39 Eric Tartour 40 Antoine Toubert 41 Marie-Noëlle Ungeheuer 42
29 CRESS - U1153 - Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle
UP13 - Université Paris 13, INRA - Institut National de la Recherche Agronomique, CNAM - Conservatoire National des Arts et Métiers [CNAM], CRESS (U1153 / UMR_A_1125 / UMR_S_1153) - Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité
Abstract : Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.
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Submitted on : Thursday, October 20, 2016 - 10:34:31 AM
Last modification on : Saturday, October 24, 2020 - 7:52:02 AM

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Darragh Duffy, Vincent Rouilly, Valentina Libri, Milena Hasan, Benoit Beitz, et al.. Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli.. Immunity, Elsevier, 2014, 40 (3), pp.436-50. ⟨10.1016/j.immuni.2014.03.002⟩. ⟨pasteur-01384537⟩

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