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DC-SIGN facilitates fusion of dendritic cells with human T-cell leukemia virus type 1-infected cells.

Abstract : Interactions between the oncogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) and dendritic cells (DCs) are poorly characterized. We show here that monocyte-derived DCs form syncytia and are infected upon coculture with HTLV-1-infected lymphocytes. We examined the role of DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN), a C-type lectin expressed in DCs, in HTLV-1-induced syncytium formation. DC-SIGN is known to bind with high affinity to various viral envelope glycoproteins, including human immunodeficiency virus (HIV) and hepatitis C virus, as well as to the cellular receptors ICAM-2 and ICAM-3. After cocultivating DCs and HTLV-1-infected cells, we found that anti-DC-SIGN monoclonal antibodies (MAbs) were able to decrease the number and size of HTLV-1-induced syncytia. Moreover, expression of the lectin in epithelial-cell lines dramatically enhanced the ability to fuse with HTLV-1-positive cells. Interestingly, in contrast to the envelope (Env) glycoproteins of HIV and other viruses, that of HTLV-1 does not bind directly to DC-SIGN. The facilitating role of the lectin in HTLV-1 syncytium formation is mediated by its interaction with ICAM-2 and ICAM-3, as demonstrated by use of MAbs directed against these adhesion molecules. Altogether, our results indicate that DC-SIGN facilitates HTLV-1 infection and fusion of DCs through an ICAM-dependent mechanism.
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Submitted on : Tuesday, September 27, 2016 - 2:49:04 PM
Last modification on : Friday, April 10, 2020 - 5:19:53 PM

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Pierre-Emmanuel Ceccaldi, Frédéric Delebecque, Marie-Christine Prevost, Arnaud Moris, Jean-Pierre Abastado, et al.. DC-SIGN facilitates fusion of dendritic cells with human T-cell leukemia virus type 1-infected cells.. Journal of Virology, American Society for Microbiology, 2006, 80 (10), pp.4771-80. ⟨10.1128/JVI.80.10.4771-4780.2006⟩. ⟨pasteur-01372637⟩

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