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Journal articles
The GTPase IFT27 is involved in both anterograde and retrograde intraflagellar transport.
Abstract : The construction of cilia and flagella depends on intraflagellar transport (IFT), the bidirectional movement of two protein complexes (IFT-A and IFT-B) driven by specific kinesin and dynein motors. IFT-B and kinesin are associated to anterograde transport whereas IFT-A and dynein participate to retrograde transport. Surprisingly, the small GTPase IFT27, a member of the IFT-B complex, turns out to be essential for retrograde cargo transport in Trypanosoma brucei. We reveal that this is due to failure to import both the IFT-A complex and the IFT dynein into the flagellar compartment. To get further molecular insight about the role of IFT27, GDP- or GTP-locked versions were expressed in presence or absence of endogenous IFT27. The GDP-locked version is unable to enter the flagellum and to interact with other IFT-B proteins and its sole expression prevents flagellum formation. These findings demonstrate that a GTPase-competent IFT27 is required for association to the IFT complex and that IFT27 plays a role in the cargo loading of the retrograde transport machinery.DOI: http://dx.doi.org/10.7554/eLife.02419.001.
Cited literature [62 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01301212
Contributor : Anne Lassailly-Bondaz <>
Submitted on : Monday, April 11, 2016 - 5:43:21 PM
Last modification on : Wednesday, December 9, 2020 - 3:05:15 PM
Long-term archiving on: : Tuesday, July 12, 2016 - 11:41:03 AM
Contributor : Anne Lassailly-Bondaz <>
Submitted on : Monday, April 11, 2016 - 5:43:21 PM
Last modification on : Wednesday, December 9, 2020 - 3:05:15 PM
Long-term archiving on: : Tuesday, July 12, 2016 - 11:41:03 AM
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