Skip to Main content Skip to Navigation
Journal articles

Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation

Abstract : Tyrosine (Y)-recombinases have evolved to deliver mechanistically different reactions on a variety of substrates, but these evolutionary transitions are poorly understood. Among them, integron integrases are hybrid systems recombining single-and double-stranded DNA partners. These reactions are asymmetric and need a replicative resolution pathway, an exception to the canonical second strand exchange model of Y-recombinases. Integron integrases possess a specific domain for this specialized pathway. Here we show that despite this, integrases are still capable of efficiently operating the ancestral second strand exchange in symmetrical reactions between double-stranded substrates. During these reactions, both strands are reactive and Holliday junction resolution can follow either pathway. A novel deep-sequencing approach allows mapping of the crossover point for the second strand exchange. The persistence of the ancestral activity in integrases illustrates their robustness and shows that innovation towards new recombination substrates and resolution pathways was a smooth evolutionary process.
Complete list of metadatas

Cited literature [57 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01292328
Contributor : Jose Escudero <>
Submitted on : Tuesday, March 22, 2016 - 6:46:43 PM
Last modification on : Monday, January 13, 2020 - 5:08:17 PM
Long-term archiving on: : Thursday, June 23, 2016 - 4:22:23 PM

File

ncomms10937.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Jose Antonio Escudero, Celine Loot, Vincent Parissi, Aleksandra Nivina, Christiane Bouchier, et al.. Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation. Nature Communications, Nature Publishing Group, 2016, 7, pp.Article number 10937. ⟨10.1038/ncomms10937⟩. ⟨pasteur-01292328⟩

Share

Metrics

Record views

425

Files downloads

415