Skip to Main content Skip to Navigation
Journal articles

Evaluation of Most-Probable-Number–PCR Method with Internal Amplification Control for the Counting of Total and Pathogenic Vibrio parahaemolyticus in Frozen Shrimps

Abstract : The most-probable-number (MPN) method is often time-consuming for the isolation, detection, and quantification of Vibrio parahaemolyticus from natural sources. MPN counting of V. parahaemolyticus bacteria usually involves the isolation of typical V. parahaemolyticus colonies on selective medium, with subsequent confirmation by biochemical identification. In this study, we evaluated the use of a PCR on MPN enrichment cultures (MPN-PCR) for the direct detection of total and pathogenic V. parahaemolyticus cells in frozen shrimp. This reaction targeted the R72H, tdh, and trh sequences. An internal amplification control was added to the samples before R72H amplification. There was an excellent correlation between the results of the two methods for artificially inoculated and natural shrimp samples. Of 36 natural samples, 28 tested positive for the presence of V. parahaemolyticus, with an MPN value of 2 | 10 21 to 9.2 | 10 1 per g. No pathogenic V. parahaemolyticus cells were detected. The test had a detection limit of one V. parahaemolyticus organism per g and was completed within two working days. These results support the use of the combination of PCR with MPN for the detection of total or potentially pathogenic V. parahaemolyticus cells in frozen shrimp.
Complete list of metadatas

Cited literature [17 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01136539
Contributor : Marie Lemesle <>
Submitted on : Friday, March 27, 2015 - 2:58:31 PM
Last modification on : Thursday, June 18, 2020 - 4:30:02 PM
Long-term archiving on: : Thursday, July 2, 2015 - 8:35:52 AM

Identifiers

Collections

Citation

S Copin, A Robert-Pillot, P Malle, M.-L Quilici, M Gay. Evaluation of Most-Probable-Number–PCR Method with Internal Amplification Control for the Counting of Total and Pathogenic Vibrio parahaemolyticus in Frozen Shrimps. Journal of Food Protection, International Association for Food Protection, 2012, 75 (1), pp.150-153. ⟨10.4315/0362-028X.JFP-11-165⟩. ⟨pasteur-01136539⟩

Share

Metrics

Record views

244