The Panhandle Formed by Influenza A and C Virus NS Non-Coding Regions Determines NS Segment Expression

Bernadette Crescenzo-Chaigne (1) , Cyril Barbezange (1) , Sylvie van Der Werf (1)

1 GMV-ARN (UMR_3569 / U-Pasteur_2) - Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses

Exchange of the extremities of the NS segment of type A and C influenza viruses in reverse genetics systems was used to assess their putative role in type specificity. Restoration of each specific proximal panhandle was mandatory to allow the rescue of viruses with heterotypic extremities. Moreover, the transcription level of the modified segment seemed to be directly affected by the distal panhandle strength. Citation: Crescenzo-Chaigne B, Barbezange C, van der Werf S (2013) The Panhandle Formed by Influenza A and C Virus NS Non-Coding Regions Determines NS Segment Expression. PLoS ONE 8(11): e81550.

Domaines: Virologie; Biologie moléculaire

Bernadette Crescenzo-Chaigne, Cyril Barbezange, Sylvie van Der Werf. The Panhandle Formed by Influenza A and C Virus NS Non-Coding Regions Determines NS Segment Expression. PLoS ONE, 2013, pp.e81550. ⟨10.1371/journal.pone.0081550⟩. ⟨pasteur-01103986⟩