Skip to Main content Skip to Navigation
Journal articles

Lineage-specific regulation of imprinted X inactivation in extraembryonic endoderm stem cells.

Abstract : BACKGROUND: Silencing of the paternal X chromosome (Xp), a phenomenon known as imprinted X-chromosome inactivation (I-XCI), characterises, amongst mouse extraembryonic lineages, the primitive endoderm and the extraembryonic endoderm (XEN) stem cells derived from it. RESULTS: Using a combination of chromatin immunoprecipitation characterisation of histone modifications and single-cell expression studies, we show that whilst the Xp in XEN cells, like the inactive X chromosome in other cell types, globally accumulates the repressive histone mark H3K27me3, a large number of Xp genes locally lack H3K27me3 and escape from I-XCI. In most cases this escape is specific to the XEN cell lineage. Importantly, the degree of escape and the genes concerned remain unchanged upon XEN conversion into visceral endoderm, suggesting stringent control of I-XCI in XEN derivatives. Surprisingly, chemical inhibition of EZH2, a member of the Polycomb repressive complex 2 (PRC2), and subsequent loss of H3K27me3 on the Xp, do not drastically perturb the pattern of silencing of Xp genes in XEN cells. CONCLUSIONS: The observations that we report here suggest that the maintenance of gene expression profiles of the inactive Xp in XEN cells involves a tissue-specific mechanism that acts partly independently of PRC2 catalytic activity.
Document type :
Journal articles
Complete list of metadatas

Cited literature [58 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01030717
Contributor : Ed. Bmc <>
Submitted on : Tuesday, July 22, 2014 - 1:18:59 PM
Last modification on : Tuesday, January 14, 2020 - 10:30:06 AM
Long-term archiving on: : Tuesday, November 25, 2014 - 11:01:18 AM

Identifiers

Citation

Sarra Merzouk, Jane Deuve, Agnès Dubois, Pablo Navarro Gil, Philip Avner, et al.. Lineage-specific regulation of imprinted X inactivation in extraembryonic endoderm stem cells.. Epigenetics & Chromatin, BioMed Central, 2014, 7 (1), pp.11. ⟨10.1186/1756-8935-7-11⟩. ⟨pasteur-01030717⟩

Share

Metrics

Record views

310

Files downloads

570