Skip to Main content Skip to Navigation
Journal articles

N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.

Abstract : Sorting of glycosylphosphatidyl-inositol--anchored proteins (GPI-APs) in polarized epithelial cells is not fully understood. Oligomerization in the Golgi complex has emerged as the crucial event driving apical segregation of GPI-APs in two different kind of epithelial cells, Madin-Darby canine kidney (MDCK) and Fisher rat thyroid (FRT) cells, but whether the mechanism is conserved is unknown. In MDCK cells cholesterol promotes GPI-AP oligomerization, as well as apical sorting of GPI-APs. Here we show that FRT cells lack this cholesterol-driven oligomerization as apical sorting mechanism. In these cells both apical and basolateral GPI-APs display restricted diffusion in the Golgi likely due to a cholesterol-enriched membrane environment. It is striking that N-glycosylation is the critical event for oligomerization and apical sorting of GPI-APs in FRT cells but not in MDCK cells. Our data indicate that at least two mechanisms exist to determine oligomerization in the Golgi leading to apical sorting of GPI-APs. One depends on cholesterol, and the other depends on N-glycosylation and is insensitive to cholesterol addition or depletion.
Document type :
Journal articles
Complete list of metadatas

Cited literature [64 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-00663149
Contributor : Marie Lemesle <>
Submitted on : Friday, February 3, 2012 - 2:44:10 PM
Last modification on : Wednesday, October 14, 2020 - 3:44:03 AM
Long-term archiving on: : Friday, May 4, 2012 - 2:20:30 AM

File

4621.full.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Naga Salaija Imjeti, Stéphanie Lebreton, Simona Paladino, Erwin de la Fuente, Alfonso Gonzalez, et al.. N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.. Molecular Biology of the Cell, American Society for Cell Biology, 2011, 22 (23), pp.4621-34. ⟨10.1091/mbc.E11-04-0320⟩. ⟨pasteur-00663149⟩

Share

Metrics

Record views

397

Files downloads

628