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Journal articles
Capsid protein identification and analysis of mature Triatoma virus (TrV) virions and naturally occurring empty particles.
Jon Agirre
1, 2
Kerman Aloria
3
Jesus M Arizmendi
2
Ibón Iloro
4
Félix Elortza
4
Rubén Sánchez-Eugenia
1, 5
Gerardo A Marti
6
Emmanuelle Neumann
7
Félix A. Rey
8
Diego M A Guérin
1, 2, *
*
Corresponding author
Félix Elortza 4
Author
Rubén Sánchez-Eugenia 1, 5
Author
Gerardo A Marti 6
Author
Emmanuelle Neumann 7
Author IdHAL : emmanuelle-neumann IdRef : https://www.idref.fr/070009627
Félix A. Rey 8
Author
Diego M A Guérin 1, 2
Correspondent author
1
Unidad de Biofísica (CSIC-UPV/EHU) (Barrio Sarriena S/N, 48940, Leioa, Bizkaia - Spain)
- CSIC-UPV/EHU (France)
2
Departamento de Bioquímica y Biologia Molecular (48940 Leioa - Spain)
- UPV/EHU - Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (Barrio Sarriena s/n, 48940 Leioa, Bizkaia Campus d'Álava : Vice-Rectorado, San Antonio 41, 01005 Vitoria ; campus de Biscaye et services centraux : Apdo 1397, 48080 Bilbao ; campus de Guipúzcoa : Vice-Rectorado, Fuenterrabia 13-1° , 20006 San Sebastián - Spain)
3
Servicio General de Proteómica (SGIker) (48940 Leioa - Spain)
- UPV/EHU - Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (Barrio Sarriena s/n, 48940 Leioa, Bizkaia Campus d'Álava : Vice-Rectorado, San Antonio 41, 01005 Vitoria ; campus de Biscaye et services centraux : Apdo 1397, 48080 Bilbao ; campus de Guipúzcoa : Vice-Rectorado, Fuenterrabia 13-1° , 20006 San Sebastián - Spain)
4
CIC-BioGUNE (Proteomic plataform, CIBERehd, Proteored, Parque Tecnológico Bizkaia, Ed800, 48160 Derio, Bizkaia - Spain)
- CIC-BioGUNE (France)
5
Fundación Biofísica Bizkaia (B Sarriena S/N, 48940 Leioa, Bizkaia - Spain)
- Fundación Biofísica Bizkaia (France)
6
Centro de Estudios Parasitológicos y de Vectores and Centro Regional de Investigaciones Científicas y Transferencia Tecnológicas La Rioja (2#584 (1900) La Plata - Argentina)
- CEPAVE - Centro de Estudios Parasitologicos y de Vectores [La Plata] ( Boulevard 120 s/n entre av. 60 y calle 64 (1900) La Plata – Buenos Aires - Argentina)
- CONICET - Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (Avda. Rivadavia 1917 - CP C1033AAJ - Cdad. de Buenos Aires - Argentina)
- UNLP - Universidad Nacional de la Plata [Argentine] (125, Güemes 124, Ensenada, Buenos Aires - Argentina)
- CIC - Comisión de Investigaciones Científicas [Buenos Aires] (Argentina)
7
IBS - UMR 5075 - Institut de biologie structurale (Institut de Biologie Structurale, 71 avenue des martyrs, CS 10090, 38044 Grenoble Cedex 9 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5075 (France)
- UGA [2016-2019] - Université Grenoble Alpes [2016-2019] (CS 40700 - 38058 Grenoble cedex - France)
- IRIG - Institut de Recherche Interdisciplinaire de Grenoble : DRF/IRIG/DBSC (France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/IRIG (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/IRIG (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
8
Virologie Structurale (25-28 rue du Docteur Roux, F-75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA3015 (France)
Abstract : Triatoma virus (TrV) is a non-enveloped +ssRNA virus belonging to the insect virus family Dicistroviridae. Mass spectrometry (MS) and gel electrophoresis were used to detect the previously elusive capsid protein VP4. Its cleavage sites were established by sequencing the N-terminus of the protein precursor and MS, and its stoichiometry with respect to the other major capsid proteins (VP1-3) was found to be 1:1. We also characterized the polypeptides comprising the naturally occurring non-infectious empty capsids, i.e., RNA-free TrV particles. The empty particles were composed of VP0-VP3 plus at least seven additional polypeptides, which were identified as products of the capsid precursor polyprotein. We conclude that VP4 protein appears as a product of RNA encapsidation, and that defective processing of capsid proteins precludes genome encapsidation.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-00545521
Contributor : Mireille Gau <>
Submitted on : Friday, December 10, 2010 - 2:34:31 PM
Last modification on : Tuesday, October 6, 2020 - 4:12:03 PM
Contributor : Mireille Gau <>
Submitted on : Friday, December 10, 2010 - 2:34:31 PM
Last modification on : Tuesday, October 6, 2020 - 4:12:03 PM
Identifiers
- HAL Id : pasteur-00545521, version 1
- DOI : 10.1016/j.virol.2010.09.034
- PUBMED : 21030058
Citation
Jon Agirre, Kerman Aloria, Jesus M Arizmendi, Ibón Iloro, Félix Elortza, et al.. Capsid protein identification and analysis of mature Triatoma virus (TrV) virions and naturally occurring empty particles.. Virology, Elsevier, 2011, 409 (1), pp.91-101. ⟨10.1016/j.virol.2010.09.034⟩. ⟨pasteur-00545521⟩
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