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Journal articles
Small molecule inhibitors of the Yersinia type III secretion system impair the development of Chlamydia after entry into host cells.
Abstract : BACKGROUND: Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epithelial cells. In this study we have analyzed the effect of these drugs on bacterial invasiveness. RESULTS: We demonstrate that INPs affect Chlamydia growth in a dose dependent manner after bacterial invasion. The efficiency of C. trachomatis L2 and C. caviae GPIC entry into host cells was not altered in the presence of INPs. In C. caviae, entry appears to proceed normally with recruitment of actin and the small GTPases Rac, Cdc42 and Arf6 to the site of bacterial entry. CONCLUSION: INPs have a strong inhibitory effect on Chlamydia growth. However, bacterial invasion is not altered in the presence of these drugs. In the light of these results, we discuss several hypotheses regarding the mode of action of INPs on type III secretion during the Chlamydia infectious cycle.
Cited literature [27 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-00409865
Contributor : Marie Lemesle Connect in order to contact the contributor
Submitted on : Friday, August 21, 2009 - 4:37:18 PM
Last modification on : Tuesday, October 19, 2021 - 10:27:20 PM
Long-term archiving on: : Tuesday, June 15, 2010 - 10:25:14 PM
Contributor : Marie Lemesle Connect in order to contact the contributor
Submitted on : Friday, August 21, 2009 - 4:37:18 PM
Last modification on : Tuesday, October 19, 2021 - 10:27:20 PM
Long-term archiving on: : Tuesday, June 15, 2010 - 10:25:14 PM
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1471-2180-9-75.pdf
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