Skip to Main content Skip to Navigation
Journal articles

Aspergillus fumigatus-induced IL-8 synthesis by respiratory epithelial cells is controlled by the PI3 kinase, p38 MAPK and ERK1/2 pathways and not by the TLR-MYD88 pathway.

Abstract : Previous studies have established that phagocytes are key cells of the pulmonary innate immune defense against A. fumigatus, an opportunistic fungus responsible of invasive pulmonary aspergillosis. Macrophages detect A. fumigatus via Toll-like receptors (TLR) 2 and 4, and respond by the MyD88-NF-B-dependent synthesis of inflammatory mediators. In the present study, we demonstrate that respiratory epithelial cells also sense A. fumigatus and participate to the host defense. Thus, the interaction of respiratory epithelial cells with germinating but not resting conidia of A. fumigatus results in interleukin (IL)-8 synthesis that is controlled by PI3 kinase, p38 MAPK and ERK1/2. Using MyD88-dominant negative transfected cells, we also show that IL-8 production is not dependent on the TLR-MyD88 pathway, although the MyD88 pathway is activated by A. fumigatus and leads to NF-B activation. Thus, our results provide evidence for the existence of two independent signalling pathways activated in respiratory epithelial cells by A. fumigatus, one which is MyD88 dependent and another that is My88 independent and involved in IL-8 synthesis.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-00320025
Contributor : Michel Chignard <>
Submitted on : Wednesday, September 10, 2008 - 10:00:26 AM
Last modification on : Friday, June 5, 2020 - 4:04:09 PM
Long-term archiving on: : Tuesday, June 28, 2011 - 4:33:29 PM

Files

Balloy_et_al_JBC26-08-08.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Viviane Balloy, Jean-Michel Sallenave, Yongzheng Wu, Lhousseine Touqui, Jean-Paul Latgé, et al.. Aspergillus fumigatus-induced IL-8 synthesis by respiratory epithelial cells is controlled by the PI3 kinase, p38 MAPK and ERK1/2 pathways and not by the TLR-MYD88 pathway.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2008, epub ahead of print. ⟨10.1074/jbc.M803149200⟩. ⟨pasteur-00320025⟩

Share

Metrics

Record views

332

Files downloads

244