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Nucleotide variation in Sabin type 2 poliovirus from an immunodeficient patient with poliomyelitis.

Abstract : The molecular and antigenic properties of a Sabin-like type 2 poliovirus, isolated from the stool samples of a 2-year-old agammaglobulinaemic child who developed paralysis 1 year after receiving the third dose of oral poliovirus vaccine, were analysed. The virus revealed 0.88 % genome variation in the VP1 region compared with the standard reference strain, compatible with replication of the virus in the intestine over approximately 1 year. The typical mutations in the 5'NCR and VP1 associated with reversion to neurovirulence for Sabin type 2 poliovirus were found. Despite this, the virus was characterized by both PCR and ELISA tests as Sabin-like and showed temperature sensitivity and neurovirulence in transgenic mice typical of the Sabin type 2 vaccine strain. Gammaglobulin replacement therapy led rapidly to virus clearance, which, when combined with treatment with the antiviral drug pleconaril, stopped virus excretion; no further virus shedding occurred. This is the first case of poliomyelitis and long-term excretion from an immunodeficient patient to be reported in Italy through the active 'Acute Flaccid Paralysis' surveillance system.
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Submitted on : Monday, September 8, 2008 - 3:51:38 PM
Last modification on : Monday, January 13, 2020 - 5:08:14 PM

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Gabriele Buttinelli, Valentina Donati, Stefano Fiore, Jill Marturano, Alessandro Plebani, et al.. Nucleotide variation in Sabin type 2 poliovirus from an immunodeficient patient with poliomyelitis.. Journal of General Virology, Microbiology Society, 2003, 84 (Pt 5), pp.1215-21. ⟨10.1099/vir.0.18974-0⟩. ⟨pasteur-00319503⟩

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