Skip to Main content Skip to Navigation
Journal articles

Expression of dengue ApoptoM sequence results in disruption of mitochondrial potential and caspase activation.

Abstract : Apoptotic cell death has been involved as a cytopathologic mechanism in response to dengue (DEN) virus infection. Little information exists about how DEN virus replication triggers apoptosis in infected cells. We reported that a nine-residue sequence of the DEN M protein referred to as ApoptoM has proapoptotic properties in transformed and tumor cells of various origins. The aim of the present study was to investigate whether ApoptoM-induced apoptosis is associated to mitochondrial dysfunction and requires caspase activation. Intracellular expression of ApoptoM provokes the disruption of the mitochondrial transmembrane potential without subsequent generation of reactive oxygen species. We showed that ApoptoM-induced apoptosis involves the activation of a caspase-like protease pathway. Caspase-3 like activity was detected in ApoptoM-expressing cells. However, there was no role for caspase-9 in ApoptoM-mediated cell death. Our data suggest that a particular mitochondrion-dependent apoptotic pathway may be involved in induction of apoptosis by ApoptoM.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-00193971
Contributor : Marie-Dominique Aytac <>
Submitted on : Wednesday, December 5, 2007 - 11:33:28 AM
Last modification on : Friday, May 15, 2020 - 6:48:02 PM

Identifiers

Collections

Citation

Adeline Catteau, Gaël Roué, Victor J Yuste, Santos A Susin, Philippe Desprès. Expression of dengue ApoptoM sequence results in disruption of mitochondrial potential and caspase activation.. Biochimie, Elsevier, 2003, 85 (8), pp.789-93. ⟨10.1016/S0300-9084(03)00139-1⟩. ⟨pasteur-00193971⟩

Share

Metrics

Record views

210