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Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

Abstract : A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.
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Submitted on : Friday, September 21, 2007 - 3:49:12 PM
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F. Colbere-Garapin, S. Chousterman, F. Horodniceanu, P. Kourilsky, A. C. Garapin. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 1979, 76 (8), pp.3755-9. ⟨10.1073/pnas.76.8.3755⟩. ⟨pasteur-00174138⟩

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