Identification of a novel coronavirus in patients with severe acute respiratory syndrome.

Christian Drosten (1) , Stephan Günther (1) , Wolfgang Preiser (2) , Sylvie van Der Werf (3) , Hans-Reinhard Brodt (4) , Stephan Becker (5) , Holger Rabenau (2) , Marcus Panning (1) , Larissa Kolesnikova (5) , Ron a M Fouchier (6) , Annemarie Berger (2) , Ana-Maria Burguière (3) , Jindrich Cinatl (2) , Markus Eickmann (5) , Nicolas Escriou (3) , Klaus Grywna (1) , Stefanie Kramme (1) , Jean-Claude Manuguerra (3) , Stefanie Müller (1) , Volker Rickerts (4) , Martin Stürmer (2) , Simon Vieth (1) , Hans-Dieter Klenk (5) , Albert D M E Osterhaus (6) , Herbert Schmitz (1) , Hans Wilhelm Doerr (2)

1 BNITM - Bernhard Nocht Institute for Tropical Medicine - Bernhard-Nocht-Institut für Tropenmedizin [Hamburg, Germany]
2 Institute of Medical Virology
3 Génétique Moléculaire des Virus Respiratoires
4 Medical Clinic III
5 Institut für Virologie
6 Institute of Virology

BACKGROUND: The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS: Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS: A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent of the nucleotide sequence). On the basis of the obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated. CONCLUSIONS: The novel coronavirus might have a role in causing SARS.

Domains: Molecular biology

Christian Drosten, Stephan Günther, Wolfgang Preiser, Sylvie van Der Werf, Hans-Reinhard Brodt, et al.. Identification of a novel coronavirus in patients with severe acute respiratory syndrome.. New England Journal of Medicine, 2003, 348 (20), pp.1967-76. ⟨10.1056/NEJMoa030747⟩. ⟨pasteur-00167033⟩