Short synthetic glycopeptides successfully induce antibody responses to carcinoma-associated Tn antigen. - Archive ouverte HAL Access content directly
Journal Articles Journal of Peptide Research Year : 2000

Short synthetic glycopeptides successfully induce antibody responses to carcinoma-associated Tn antigen.

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Abstract

Glycopeptides containing a tumor-associated carbohydrate antigen (mono-, tri- or hexa-Tn antigen) as a B-cell epitope and a CD4+ T-cell epitope (PV: poliovirus or TT: tetanus toxin) were prepared for immunological studies. Several Tn antigen residues [FmocSer/Thr (alpha-GalNAc)-OH] were successively incorporated into the peptide sequence with unprotected carbohydrate groups. The tri- and hexa-Tn glycopeptides were recognized by MLS128, a Tn-specific monoclonal antibody. The position of the tri-Tn motif in the peptide sequence and the peptide backbone itself do not alter its antigenicity. As demonstrated by both ELISA and FACS analysis, the glycopeptides induced high titers of anti-Tn antibodies in mice, in the absence of a carrier molecule. In addition, the generated antibodies recognized the native Tn antigen on cancer cells. The antibody response obtained with a D-(Tn3)-PV glycopeptide containing three alpha-GalNAc-D-serine residues is similar that obtained with the Tn6-PV glycopeptide. These results demonstrate that short synthetic glycopeptides are able to induce anticancer antibody responses.

Dates and versions

pasteur-00166856 , version 1 (10-08-2007)

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S. Vichier-Guerre, R. Lo-Man, S. Bay, E. Deriaud, H. Nakada, et al.. Short synthetic glycopeptides successfully induce antibody responses to carcinoma-associated Tn antigen.. Journal of Peptide Research, 2000, 55 (2), pp.173-80. ⟨10.1034/j.1399-3011.2000.00167.x⟩. ⟨pasteur-00166856⟩

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