Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR - Institut Pasteur Access content directly
Journal Articles Nature Methods Year : 2018

Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Jonathan Marvin
Bart Borghuis

Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.

Dates and versions

pasteur-04092458 , version 1 (09-05-2023)

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Jonathan Marvin, Benjamin Scholl, Daniel Wilson, Kaspar Podgorski, Abbas Kazemipour, et al.. Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nature Methods, 2018, 15 (11), pp.936-939. ⟨10.1038/s41592-018-0171-3⟩. ⟨pasteur-04092458⟩
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