Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Jonathan Marvin (1) , Benjamin Scholl (2) , Daniel Wilson (2) , Kaspar Podgorski (1) , Abbas Kazemipour (1) , Johannes Alexander Müller (3) , Susanne Schoch (3) , Francisco José Urra Quiroz (4) , Nelson Rebola (4) , Huan Bao (5) , Justin Little (1) , Ariana Tkachuk (1) , Edward Cai (1) , Adam Hantman (1) , Samuel S.-H. Wang (6) , Victor Depiero (7) , Bart Borghuis (7) , Edwin Chapman (5) , Dirk Dietrich (3) , David Digregorio (4) , David Fitzpatrick (2) , Loren Looger (1)

1 HHMI - Howard Hughes Medical Institute
2 MPFI - Max Planck Florida Institute for Neuroscience
3 Rheinische Friedrich-Wilhelms-Universität Bonn
4 Dynamique des synapses et des circuits neuronaux - Synapse and circuit dynamics
5 University of Wisconsin-Madison
6 Princeton University
7 University of Louisville

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.

Domaines: Sciences du Vivant [q-bio]

Jonathan Marvin, Benjamin Scholl, Daniel Wilson, Kaspar Podgorski, Abbas Kazemipour, et al.. Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nature Methods, 2018, 15 (11), pp.936-939. ⟨10.1038/s41592-018-0171-3⟩. ⟨pasteur-04092458⟩