Crystallization of the catalytic domain of murine terminal deoxynucleotidyl transferase

N. Sukumar (1) , J. Boulé (2) , N. Expert-Bezançon (1) , N. Jourdan (1) , J. Lescar (3) , F. Rougeon (2) , C. Papanicolaou (2) , M. Delarue (1)

1 Biochimie Structurale
2 Génétique et Biochimie du Développement
3 ESRF - European Synchrotron Radiation Facility

The catalytic domain of murine terminal deoxynucleotidyl transferase (TdT) has been crystallized in the space group P2(1)2(1)2(1), with unit-cell parameters a = 47.1, b = 86.2, c = 111.7 A. The crystals diffract to a resolution of 2.4 A using synchrotron radiation and a full data set has been collected from the native crystals. The enzyme was shown to be active in the crystalline state.

Keywords: DNA polymerases; terminal deoxynucleotidyl transferase

Domains: Life Sciences [q-bio]; Chemical Sciences

N. Sukumar, J. Boulé, N. Expert-Bezançon, N. Jourdan, J. Lescar, et al.. Crystallization of the catalytic domain of murine terminal deoxynucleotidyl transferase. Acta crystallographica Section D : Structural biology [1993-..], 2000, 56 (12), pp.1662-1664. ⟨10.1107/s090744490001297x⟩. ⟨pasteur-04089397⟩