Chapter 1 - Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators

Marie Juzans (1, 2) , Céline Cuche (1) , Vincenzo Di Bartolo (1) , Andrés Alcover (1)

1 Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology
2 Perelman School of Medicine

Immunological synapse formation results from a profound T cell polarization process that involves the coordinated action of the actin and microtubule cytoskeleton, and the intracellular traffic of several vesicular organelles. T cell polarization is key for both T cell activation lead- ing to T cell proliferation and differentiation, and for T cell effector functions such as polarized secretion of cytokines by helper T cells, or polarized delivery of lytic granules by cytotoxic T cells. Efficient targeting of lytic granules by cytotoxic T cells is a crucial event for the con- trol and elimination of infected or tumor cells. Understanding how lytic granule delivery is regulated and quantifying its efficiency under physiological and pathological conditions may help to improve immune responses against infection and cancer.

Mots clés: Immunological synapse; Cytotoxic T cells; Lytic granules; Polarized released; TIRF microscopy; Polarity regulators

Domaines: Biologie cellulaire; Immunologie

Marie Juzans, Céline Cuche, Vincenzo Di Bartolo, Andrés Alcover. Chapter 1 - Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators. Clément Thomas; Lorenzo Galluzzi. The Immunological Synapse Part A, 173, Elsevier, pp.1-13, 2022, Methods in Cell Biology, 978-0-323-90154-3. ⟨10.1016/bs.mcb.2022.07.016⟩. ⟨pasteur-03992601⟩