Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures

Manfred Weidmann (1) , Amadou Sall (2) , Jean-Claude Manuguerra (3) , Lamine Koivogui (4) , Aime Adjami (5) , Faye Fatou Traoré (6) , Kjell-Olof Hedlund (7) , Gunnel Lindegren (7) , Ali Mirazimi (8)

1 UMG - University Medical Center Göttingen
2 Institut Pasteur de Dakar
3 CIBU - Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats
4 Université Gamal Abdel Nasser de Conakry
5 WHO Burkina Faso - World Health Organizatin [Ouagadougou, Burkina Faso]
6 Fondation Mérieux
7 SMI - Smittskydds Institutet - Swedish Institute for Infectious Disease Control [Solna, Sweden]
8 Department of Microbiology, Tumor and Cell Biology

Information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV), of the Arenaviridae, Filoviridae, Bunyaviridae, and Flavivridae virus families by performing quantitative analysis of cell culture supernatants by (i) electron microscopy for the quantification of virus particles, (ii) quantitative real time PCR for the quantification of genomes, and (iii) determination of focus forming units by coating fluorescent antibodies to infected cell monolayers for the quantification of virus infectivity.The comparative analysis revealed that filovirus and RVFV replication results in a surplus of genomes but varying degrees of packaging efficiency and infectious particles. More efficient replication and packaging was observed for Lassa virus, and Dengue virus resulting in a better yield of infectious particles while, YFV turned out to be most efficient with only 4 particles inducing one FFU. For Crimean-Congo haemorrhagic fever virus (CCHFV) a surplus of empty shells was observed with only one in 24 particles equipped with a genome. The complete particles turned out to be extraordinarily infectious.

Domaines: Sciences du Vivant [q-bio]

Manfred Weidmann, Amadou Sall, Jean-Claude Manuguerra, Lamine Koivogui, Aime Adjami, et al.. Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures. Virology Journal, 2011, 8, pp.81. ⟨10.1186/1743-422X-8-81⟩. ⟨pasteur-03915094⟩