Single mRNA Molecule Detection in Drosophila - Archive ouverte HAL Access content directly
Book Sections Year : 2018

Single mRNA Molecule Detection in Drosophila

(1) , (2)
1
2

Abstract

Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.

Dates and versions

pasteur-03869357 , version 1 (24-11-2022)

Identifiers

Cite

Shawn Little, Thomas Gregor. Single mRNA Molecule Detection in Drosophila. RNA Detection, 1649, Springer New York, pp.127-142, 2018, Methods in Molecular Biology, ⟨10.1007/978-1-4939-7213-5_8⟩. ⟨pasteur-03869357⟩

Collections

PASTEUR
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More