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Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere

Abstract : A facultative anaerobic, Gram-stain-positive, endospore-forming bacterium, isolated from the rhizosphere of maize roots ( Zea mays ), was taxonomically studied. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-79 T clustered only loosely with Neobacillus species and showed the highest similarity to Neobacillus soli (97.9%). The 16S rRNA gene sequence similarities to the sequences of the type strains of other Neobacillus species were 97.5 % and below. Chemotaxonomic features supported the grouping of the strain to the Neobacillus group, e.g. the major fatty acids were C 15 : 0 anteiso, C 15 : 0 iso and C 16 : 0 , the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid, the major quinone was menaquinone MK-7, and major compound in the polyamine pattern was spermidine. However, the JJ-79 T genome assembly did not share most of the 11 conserved signature indels that are indicative of the genus Neobacillus . The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the JJ-79 T genome assembly and those of the closest relative Bacillaceae type strains were <71, <71 and <25 %, respectively. Physiological and biochemical test results were also different from those of the most closely related Bacillaceae species. As a consequence, JJ-79 T represents a novel genus for which we propose the name Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., with JJ-79 T (=CIP 111885 T =CCM 9045 T ) as the type strain.
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Submitted on : Thursday, September 29, 2022 - 10:41:39 AM
Last modification on : Wednesday, October 5, 2022 - 3:20:22 AM

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Peter Kämpfer, Stefanie Glaeser, Hans-Jürgen Busse, John Mcinroy, Dominique Clermont, et al.. Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere. International Journal of Systematic and Evolutionary Microbiology, 2022, 72 (5), ⟨10.1099/ijsem.0.005367⟩. ⟨pasteur-03791340⟩

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