Complete Genome Sequence of Bradyrhizobium sp. Strain C-145, a Nitrogen-Fixing Rhizobacterium Used as a Peanut Inoculant in Argentina
Résumé
We present the complete genome sequence of Bradyrhizobium sp. strain C-145, one of the most widely used nitrogen-fixing rhizobacteria for inoculating peanut crops in Argentina. The genome consists of 9.53 Mbp in a single circular chromosome and was determined using a hybrid long-and short-read assembly approach. T he symbiosis between legumes and bacteria from the Bradyrhizobium genus is one of the most efficient and economically important worldwide (1). Inoculation of soybean and peanut crops with effective bradyrhizobia is an environmentally friendly alternative to chemical fertilization (2-4). The peanut-nodulating strain Bradyrhizobium sp. strain TAL 1371 (NifTAL code) was acquired by the Instituto Nacional de Tecnología Agropecuaria (INTA; Argentina) from the University of Texas (5). After being evaluated and reisolated from peanut cultivars, it was renamed Bradyrhizobium sp. strain C-145 and became the recommended choice for peanut inoculation. In symbiosis with this legume, it outperforms other Bradyrhizobium sp. strains in terms of nitrogen-fixing ability, productivity, and environmental competitiveness (4, 6-8). This is particularly relevant given the prominence of peanut cultivation in central Argentina (9). Knowledge of the strain's genome is crucial for maintaining and developing further the current agricultural model. To date, although 581 genome assemblies are registered in NCBI for Bradyrhizobium strains, most are draft sequences. No genome data were available until now for strains used in commercial peanut inoculants. Here, we introduce the complete genome sequence of Bradyrhizobium sp. C-145. A pure culture of the strain, provided by INTA, was grown in liquid yeast extract-mannitol medium (5). This was the source for the total DNA, obtained using a DNeasy blood and tissue kit (Qiagen) for Illumina sequencing and using a Promega Wizard high-molecular-weight (HMW) DNA extraction kit (Promega) for Oxford Nanopore Technologies sequencing. Illumina sequencing was performed on the P2M (Plateforme de Microbiologie Mutualisée) platform at Institut Pasteur. The library was prepared using a Nextera XT DNA library preparation kit and then sequenced on an Illumina NextSeq 500 instrument in paired-end (PE) 150-bp read configuration. Nanopore sequencing was carried out at the Oxford Genomics Centre. The sample was processed using both an Oxford Nanopore Technologies rapid barcoding Editor
Domaines
Microbiologie et Parasitologie
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