Skip to Main content Skip to Navigation
Journal articles

A multi-adenylate cyclase regulator at the flagellar tip controls African trypanosome transmission

Abstract : Abstract Signaling from ciliary microdomains controls developmental processes in metazoans. Trypanosome transmission requires development and migration in the tsetse vector alimentary tract. Flagellar cAMP signaling has been linked to parasite social motility (SoMo) in vitro, yet uncovering control of directed migration in fly organs is challenging. Here we show that the composition of an adenylate cyclase (AC) complex in the flagellar tip microdomain is essential for tsetse salivary gland (SG) colonization and SoMo. Cyclic AMP response protein 3 (CARP3) binds and regulates multiple AC isoforms. CARP3 tip localization depends on the cytoskeletal protein FLAM8. Re-localization of CARP3 away from the tip microdomain is sufficient to abolish SoMo and fly SG colonization. Since intrinsic development is normal in carp3 and flam8 knock-out parasites, AC complex-mediated tip signaling specifically controls parasite migration and thereby transmission. Participation of several developmentally regulated receptor-type AC isoforms may indicate the complexity of the in vivo signals perceived.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03779404
Contributor : Brice Rotureau Connect in order to contact the contributor
Submitted on : Friday, September 16, 2022 - 5:05:08 PM
Last modification on : Wednesday, September 21, 2022 - 3:48:44 AM

File

s41467-022-33108-z.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Sabine Bachmaier, Giacomo Giacomelli, Estefanía Calvo-Alvarez, Larissa Rezende Vieira, Jan van den Abbeele, et al.. A multi-adenylate cyclase regulator at the flagellar tip controls African trypanosome transmission. Nature Communications, Nature Publishing Group, 2022, 13 (1), pp.5445. ⟨10.1038/s41467-022-33108-z⟩. ⟨pasteur-03779404⟩

Share

Metrics

Record views

0

Files downloads

0