The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics

Youcef Shahali (1) , Hélène Sénéchal (2) , Pascal Poncet (2, 3)

1 AREEO - Agricultural Research, Education and Extension Organisation
2 CHU Trousseau [APHP]
3 Centre d'Innovation et Recherche Technologique / Center for Innovation and Technological Research

The recent progress of proteomic protocols led to more efficient protein extraction and concentration procedures to remove nonprotein interfering compounds present in the starting material and to increase the concentration of underrepresented proteins. Combinatorial hexapeptide ligand libraries (CPLL) were recently applied to both plant- and animal-derived tissues for capturing the low- and very low-abundance allergens. Several IgE-binding proteins which were previously absent or poorly represented by using conventional proteomics tools have been detected and characterized through a CPLL-based approach. In the present chapter, a protocol based on improved protein extraction and enrichment by CPLL, allowing the immunochemical characterization of several “hidden allergens” in cypress pollen, is described in detail.

Mots clés: Hexapeptide ligand libraries; Low-abundance allergens; Pollen allergens; Mass spectrometry; Proteomics

Domaines: Sciences du Vivant [q-bio]; Biochimie [q-bio.BM]; Santé; Allergologie

Youcef Shahali, Hélène Sénéchal, Pascal Poncet. The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics. Functional Proteomics, 1871, Springer New York; Humana Press, pp.393-403, 2018, Methods in Molecular Biology, 978-1-4939-8814-3. ⟨10.1007/978-1-4939-8814-3_21⟩. ⟨pasteur-03718451⟩