Skip to Main content Skip to Navigation
Journal articles

Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours

Abstract : Sepsis is a life-threatening condition induced by a deregulated host response to severe infection. Post-sepsis syndrome includes long-term psychiatric disorders, such as persistent anxiety and post-traumatic stress disorder, whose neurobiological mechanisms remain unknown. Using a reference mouse model of sepsis, we showed that mice that recovered from sepsis further developed anxiety-related behaviours associated with an exaggerated fear memory. In the brain, sepsis induced an acute pathological activation of a specific neuronal population of the central nucleus of the amygdala, which projects to the ventral bed nucleus of the stria terminalis. Using viral-genetic circuit tracing and in vivo calcium imaging, we observed that sepsis induced persistent changes in the connectivity matrix and in the responsiveness of these central amygdala neurons projecting to the ventral bed nucleus of the stria terminalis. The transient and targeted silencing of this subpopulation only during the acute phase of sepsis with a viral pharmacogenetic approach, or with the anti-epileptic and neuroprotective drug levetiracetam, prevented the subsequent development of anxiety-related behaviours. Specific inhibition of brain anxiety and fear circuits during the sepsis acute phase constitutes a preventive approach to preclude the post-infection psychiatric outcomes.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03700519
Contributor : gabriel lepousez Connect in order to contact the contributor
Submitted on : Tuesday, June 21, 2022 - 11:25:39 AM
Last modification on : Sunday, June 26, 2022 - 2:17:50 AM

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Collections

Citation

Lena Bourhy, Aurélien Mazeraud, Luis Costa, Jarod Levy, Damien Rei, et al.. Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours. Brain - A Journal of Neurology , Oxford University Press (OUP), 2022, 145 (4), pp.1391-1409. ⟨10.1093/brain/awab475⟩. ⟨pasteur-03700519⟩

Share

Metrics

Record views

0

Files downloads

0