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RNase E and HupB dynamics foster mycobacterial cell homeostasis and fitness

Abstract : RNA turnover is a primary source of gene expression variation, in turn promoting cellular adaptation. Mycobacteria leverage reversible mRNA stabilization to endure hostile conditions. Although RNase E is essential for RNA turnover in several species, its role in mycobacterial single-cell physiology and functional phenotypic diversification remains unexplored. Here, by integrating live-single-cell and quantitative-mass-spectrometry approaches, we show that RNase E forms dynamic foci, which are associated with cellular homeostasis and fate, and we discover a versatile molecular interactome. We show a likely interaction between RNase E and the nucleoid-associated protein HupB, which is particularly pronounced during drug treatment and infection, where phenotypic diversity increases. Disruption of RNase E expression affects HupB levels, impairing Mycobacterium tuberculosis growth homeostasis during treatment, intracellular replication, and host spread. Our work lays the foundation for targeting the RNase E and its partner HupB, aiming to undermine M. tuberculosis cellular balance, diversification capacity, and persistence.
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Contributor : Virginie PORTELETTE Connect in order to contact the contributor
Submitted on : Monday, June 13, 2022 - 11:08:50 AM
Last modification on : Friday, August 5, 2022 - 12:03:02 PM


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Distributed under a Creative Commons Attribution 4.0 International License




Anna Griego, Thibaut Douché, Quentin Giai Gianetto, Mariette Matondo, Giulia Manina. RNase E and HupB dynamics foster mycobacterial cell homeostasis and fitness. iScience, 2022, 25 (5), pp.104233. ⟨10.1016/j.isci.2022.104233⟩. ⟨pasteur-03693876⟩



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