Skip to Main content Skip to Navigation
Preprints, Working Papers, ...

Neutrophils degranulate GAG-containing proteoglycofili, which block Shigella growth and degrade virulence factors

Abstract : Summary paragraph Neutrophil degranulation plays a central role in their ability to kill pathogens but also to stimulate other immune cells 1–3 . Here we show that neutrophil degranulation, induced in hypoxia or upon Shigella infection in vitro and in vivo , leads to the release of polymers called neutrophil Proteoglycofili (PGF). PGF are mainly composed of granular proteins (myeloperoxidase, elastase, lactoferrin, cathelicidin, albumin) pre-stored in various types of granules, and chondroitin sulfate. PGF individual fibers have a diameter of 43.9 ± 20.3 nm and. They secreted by viable neutrophils and do not contain DNA, as opposed to NETs which contains also granular proteins, chondroitin sulfate in addition to chromatin, released upon neutrophil disintegration and cell death. We demonstrated that PGF block the growth of Shigella and other bacteria and degrade Shigella virulence factors. The degradation of the chondroitin sulfate polymers with testes hyaluronidases destabilizes PGF ultrastructure and abolishes its antimicrobial activity. Our results provide novel insights in the neutrophil degranulation process and open new doors for the investigation of PGF contribution to cytokines concentration gradient formation and adaptive immune cells activation. Further investigations are required to better appreciate the importance of this “sterile blaster” in infectious or inflammatory diseases.
Document type :
Preprints, Working Papers, ...
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03691520
Contributor : Jean-Yves Tinevez Connect in order to contact the contributor
Submitted on : Thursday, June 9, 2022 - 10:46:52 AM
Last modification on : Saturday, June 25, 2022 - 3:44:10 AM

Licence

Copyright

Identifiers

Collections

Citation

Antonin André, Marina Valente Barroso, Jurate Skerniskyte, Mélina Siegwald, Vanessa Paul, et al.. Neutrophils degranulate GAG-containing proteoglycofili, which block Shigella growth and degrade virulence factors. 2022. ⟨pasteur-03691520⟩

Share

Metrics

Record views

0

Files downloads

0