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RNA Sequencing Reveals Widespread Transcription of Natural Antisense RNAs in Entamoeba Species

Abstract : Entamoeba is a genus of Amoebozoa that includes the intestine-colonizing pathogenic species Entamoeba histolytica. To understand the basis of gene regulation in E. histolytica from an evolutionary perspective, we have profiled the transcriptomes of its closely related species E. dispar, E. moshkovskii and E. invadens. Genome-wide identification of transcription start sites (TSS) and polyadenylation sites (PAS) revealed the similarities and differences of their gene regulatory sequences. In particular, we found the widespread initiation of antisense transcription from within the gene coding sequences is a common feature among all Entamoeba species. Interestingly, we observed the enrichment of antisense transcription in genes involved in several processes that are common to species infecting the human intestine, e.g., the metabolism of phospholipids. These results suggest a potentially conserved and compact gene regulatory system in Entamoeba.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03682595
Contributor : Damien Mornico Connect in order to contact the contributor
Submitted on : Tuesday, May 31, 2022 - 10:58:58 AM
Last modification on : Friday, June 3, 2022 - 2:19:16 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Damien Mornico, Chung-Chau Hon, Mikael Koutero, Christian Weber, Jean-yves J.-y. Coppee, et al.. RNA Sequencing Reveals Widespread Transcription of Natural Antisense RNAs in Entamoeba Species. Microorganisms, MDPI, 2022, 10 (2), pp.396. ⟨10.3390/microorganisms10020396⟩. ⟨pasteur-03682595⟩

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