APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome

Pierre Khalfi (1, 2, 3) , Rodolphe Suspène (1, 3) , Vincent Caval (3, 1) , Valérie Thiers (3, 1) , Guillaume Beauclair (4) , Agnès Marchio (5, 6) , Claudine Bekondi (7) , Marie Amougou Atsama (8) , Serge-Magloire Camengo-Police (9) , Dominique Noah Noah (10) , Richard Njouom (8) , Hervé Blanc (11) , Thomas Vallet (11) , Marco Vignuzzi (11) , Pascal Pineau (6) , Jean-Pierre Vartanian (3)

1 CNRS-UMR3569 - Virologie
2 CEA - Commissariat à l'énergie atomique et aux énergies alternatives
3 Département de Virologie - Department of Virology
4 I2BC - Institut de Biologie Intégrative de la Cellule
5 IP - Institut Pasteur [Paris]
6 Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis
7 Institut Pasteur de Bangui
8 Centre Pasteur du Cameroun
9 Hôpital Central de Yaoundé [Yaoundé]
10 Hôpital de l'Amitié [Bangui]
11 Populations virales et Pathogenèse - Viral Populations and Pathogenesis

Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA processivity of the enzyme. In this study, we demonstrated in culture and in infected patients that hepatitis B virus (HBV) could be edited by APOBEC3CS188I. Using next-generation sequencing, we demonstrated that APOBEC3CS188I led to enhanced editing activity in 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of this enzyme, which could be used to determine its impact on HBV or nuclear DNA.

Mots clés: APOBEC3C; Africa; Hepatitis B virus; editing; nucleotide context; patients; polymorphism

Domaines: Virologie

Pierre Khalfi, Rodolphe Suspène, Vincent Caval, Valérie Thiers, Guillaume Beauclair, et al.. APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome. Journal of Infectious Diseases, 2022, pp.jiac003. ⟨10.1093/infdis/jiac003⟩. ⟨pasteur-03671452⟩