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Journal articles
Bat coronaviruses related to SARS-CoV-2 and infectious for human cells
Sarah Temmam
1, 2
Khamsing Vongphayloth
3
Eduard Baquero
4
Sandie Munier
5
Massimiliano Bonomi
6
Béatrice Regnault
1, 2
Bounsavane Douangboubpha
7
Yasaman Karami
6
Delphine Chrétien
1, 2
Daosavanh Sanamxay
7
Vilakhan Xayaphet
7
Phetphoumin Paphaphanh
7
Vincent Lacoste
3
Somphavanh Somlor
3
Khaithong Lakeomany
3
Nothasin Phommavanh
3
Philippe Pérot
1, 2
Océane Dehan
5, 8
Faustine Amara
5
Flora Donati
5, 8
Thomas Bigot
1, 9
Michael Nilges
6
Félix Rey
4
Sylvie van der Werf
5, 8
Paul Brey
3
Marc Eloit
1, 2, 10, *
*
Corresponding author
Sarah Temmam 1, 2
Author IdHAL : sarah-temmam
Khamsing Vongphayloth 3
Author
Eduard Baquero 4
Author
Sandie Munier 5
Author
Massimiliano Bonomi 6
Author IdHAL : massimiliano-bonomi ORCID : https://orcid.org/0000-0002-7321-0004
Béatrice Regnault 1, 2
Author
Bounsavane Douangboubpha 7
Author
Yasaman Karami 6
Author ORCID : https://orcid.org/0000-0001-8413-2665
Delphine Chrétien 1, 2
Author
Daosavanh Sanamxay 7
Author
Vilakhan Xayaphet 7
Author
Phetphoumin Paphaphanh 7
Author
Vincent Lacoste 3
Author IdHAL : vincent-lacoste ORCID : https://orcid.org/0000-0002-3173-4053
Somphavanh Somlor 3
Author
Khaithong Lakeomany 3
Author
Nothasin Phommavanh 3
Author
Philippe Pérot 1, 2
Author Employer institution : Institut Pasteur [Paris] IdHAL : philippe-perot ORCID : https://orcid.org/0000-0002-5194-8200
Océane Dehan 5, 8
Author
Faustine Amara 5
Author
Flora Donati 5, 8
Author IdHAL : flora-donati ORCID : https://orcid.org/0000-0003-2862-9751
Thomas Bigot 1, 9
Author IdHAL : thomas-bigot ORCID : https://orcid.org/0000-0001-8504-4675
Michael Nilges 6
Author ORCID : https://orcid.org/0000-0002-1451-8092
Félix Rey 4
Author ORCID : https://orcid.org/0000-0002-9953-7988
Sylvie van der Werf 5, 8
Author ORCID : https://orcid.org/0000-0002-1148-4456
Paul Brey 3
Author
Marc Eloit 1, 2, 10
Correspondent author Employer institution : École nationale vétérinaire - Alfort IdHAL : marceloitpasteurfr
1
Découverte de pathogènes – Pathogen discovery (Département de virologie, 25-28 rue du Docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- UPC - Université Paris Cité (85 boulevard Saint-Germain 75006 Paris - France)
2
CCOIE-OIECC - Centre Collaborateur de l'OIE de Détection et identification chez l’homme des pathogènes animaux émergents et développement d’outils pour leur diagnostic / Collaborating Center for the Detection and identification in humans of emerging animal pathogens and development of tools for their diagnoses (25-28 rue du Docteur Roux - 75724 Paris Cedex 15 – France - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- OIE-WAHIS - Organisation Mondiale de la Santé Animale / World Animal Health Information System (Siège mondial 12, rue de Prony, 75017 Paris - France)
- UPC - Université Paris Cité (85 boulevard Saint-Germain 75006 Paris - France)
3
Institut Pasteur du Laos (Samsenthai Road, Ban Kao-Gnot, Sisattanak district, P.O Box 3560, Vientiane, Lao PDR. - Laos)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
4
Virologie Structurale - Structural Virology (Département de Virologie - 25-28, rue du docteur Roux 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3569 (France)
- UPC - Université Paris Cité (85 boulevard Saint-Germain 75006 Paris - France)
5
GMV-ARN (UMR_3569 / U-Pasteur_2) - Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (Département de Virologie, 25-28 rue du docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR_3569 (France)
- UPC - Université Paris Cité : U-Pasteur_2 (85 boulevard Saint-Germain 75006 Paris - France)
6
Bioinformatique structurale - Structural Bioinformatics (Département de Biologie structurale et Chimie, 25-28, rue du Docteur Roux - 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3528 (France)
- UPC - Université Paris Cité (85 boulevard Saint-Germain 75006 Paris - France)
7
National University of Laos (Laos)
8
CNR - Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (Unité de Génétique Moléculaire des Virus à ARN
25-28, rue du Docteur Roux - 75724 PARIS CEDEX 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- UPC - Université Paris Cité (85 boulevard Saint-Germain 75006 Paris - France)
9
Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB (Département de biologie computationnelle (ex C3BI), 25-28 rue du Docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- UPC - Université Paris Cité (85 boulevard Saint-Germain 75006 Paris - France)
10
ENVA - École nationale vétérinaire - Alfort (7 avenue du Général de Gaulle - 94700 Maisons-Alfort - France)
Abstract : The animal reservoir of SARS-CoV-2 is unknown despite reports of SARS-CoV-2-related viruses in Asian Rhinolophus bats1-4, including the closest virus from R. affinis, RaTG13 (refs. 5,6), and pangolins7-9. SARS-CoV-2 has a mosaic genome, to which different progenitors contribute. The spike sequence determines the binding affinity and accessibility of its receptor-binding domain to the cellular angiotensin-converting enzyme 2 (ACE2) receptor and is responsible for host range10-12. SARS-CoV-2 progenitor bat viruses genetically close to SARS-CoV-2 and able to enter human cells through a human ACE2 (hACE2) pathway have not yet been identified, although they would be key in understanding the origin of the epidemic. Here we show that such viruses circulate in cave bats living in the limestone karstic terrain in northern Laos, in the Indochinese peninsula. We found that the receptor-binding domains of these viruses differ from that of SARS-CoV-2 by only one or two residues at the interface with ACE2, bind more efficiently to the hACE2 protein than that of the SARS-CoV-2 strain isolated in Wuhan from early human cases, and mediate hACE2-dependent entry and replication in human cells, which is inhibited by antibodies that neutralize SARS-CoV-2. None of these bat viruses contains a furin cleavage site in the spike protein. Our findings therefore indicate that bat-borne SARS-CoV-2-like viruses that are potentially infectious for humans circulate in Rhinolophus spp. in the Indochinese peninsula.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03661247
Contributor : Sarah Temmam Connect in order to contact the contributor
Submitted on : Thursday, May 19, 2022 - 11:23:55 AM
Last modification on : Thursday, May 19, 2022 - 11:31:45 AM
Contributor : Sarah Temmam Connect in order to contact the contributor
Submitted on : Thursday, May 19, 2022 - 11:23:55 AM
Last modification on : Thursday, May 19, 2022 - 11:31:45 AM
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Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License
Identifiers
- HAL Id : pasteur-03661247, version 1
- DOI : 10.1038/s41586-022-04532-4
- PUBMED : 35172323
Collections
PASTEUR | UP-SCIENCES | UNIV-PARIS | ENVA | RIIP_LAOS | CNRS | RIIP | ANR
Citation
Sarah Temmam, Khamsing Vongphayloth, Eduard Baquero, Sandie Munier, Massimiliano Bonomi, et al.. Bat coronaviruses related to SARS-CoV-2 and infectious for human cells. Nature, Nature Publishing Group, 2022, 604 (7905), pp.330-336. ⟨10.1038/s41586-022-04532-4⟩. ⟨pasteur-03661247⟩
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