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Azorhizobium caulinodans P II and GlnK Proteins Control Nitrogen Fixation and Ammonia Assimilation

Nathalie Michel-Reydellet 1 1, *
* Corresponding author
Abstract : We herein report that Azorhizobium caulinodans P II and GlnK are not necessary for glutamine synthetase (GS) adenylylation whereas both proteins are required for complete GS deadenylylation. The disruption of both glnB and glnK resulted in a high level of GS adenylylation under the condition of nitrogen fixation, leading to ammonium excretion in the free-living state. P II and GlnK also controlled nif gene expression because NifA activated nifH transcription and nitrogenase activity was derepressed in glnB glnK double mutants, but not in wild-type bacteria, grown in the presence of ammonia.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03659858
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Submitted on : Thursday, May 5, 2022 - 1:47:08 PM
Last modification on : Friday, May 6, 2022 - 3:38:37 AM

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Nathalie Michel-Reydellet, . Azorhizobium caulinodans P II and GlnK Proteins Control Nitrogen Fixation and Ammonia Assimilation. Journal of Bacteriology, American Society for Microbiology, 1999, 181 (8), pp.2655-2658. ⟨10.1128/JB.181.8.2655-2658.1999⟩. ⟨pasteur-03659858⟩

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