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Broadly neutralizing anti-HIV-1 antibodies tether viral particles at the surface of infected cells

Abstract : Broadly neutralizing antibodies (bNAbs) targeting the HIV-1 envelope glycoprotein (Env) are promising molecules for therapeutic or prophylactic interventions. Beyond neutralization, bNAbs exert Fc-dependent functions including antibody-dependent cellular cytotoxicity and activation of the complement. Here, we show that a subset of bNAbs targeting the CD4 binding site and the V1/V2 or V3 loops inhibit viral release from infected cells. We combined immunofluorescence, scanning electron microscopy, transmission electron microscopy and immunogold staining to reveal that some bNAbs form large aggregates of virions at the surface of infected cells. This activity correlates with the capacity of bNAbs to bind to Env at the cell surface and to neutralize cell-free viral particles. We further show that antibody bivalency is required for viral retention, and that aggregated virions are neutralized. We have thus identified an additional antiviral activity of bNAbs, which block HIV-1 release by tethering viral particles at the surface of infected cells.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03654220
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Submitted on : Thursday, April 28, 2022 - 2:33:34 PM
Last modification on : Friday, August 5, 2022 - 12:03:10 PM
Long-term archiving on: : Friday, July 29, 2022 - 7:22:28 PM

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Jérémy Dufloo, Cyril Planchais, Stéphane Frémont, Valerie Lorin, Florence Guivel-Benhassine, et al.. Broadly neutralizing anti-HIV-1 antibodies tether viral particles at the surface of infected cells. Nature Communications, Nature Publishing Group, 2022, 13 (1), pp.630. ⟨10.1038/s41467-022-28307-7⟩. ⟨pasteur-03654220⟩

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